Browsing by Author "Criste, Adriana"
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ItemDetermination of changes in the microbial and chemical composition of Taga cheese during maturation(Public Library of Science, 2020-12-03) Criste, Adriana; Copolovici, Lucian; Copolovici, Dana; Kovacs, Melinda; Madden, Robert H.; Corcionivoschi, Nicolae; Gundogdu, Ozan; Berchez, Mihaela; Urcan, Adriana CristinaȚaga cheese is a traditional Romanian smear-ripened cheese made from bovine milk and identified with the name of the village and caves where it is produced. As no previously reported microbiological and chemical studies have been undertaken on this product, this research aimed to investigate the microbiological and biochemical characteristics which ensure the uniqueness of Țaga cheese during the ripening process, to inform producers as to key quality determinants. Cheese samples, consisting of retail blocks, were collected on days 2, 5, 12, 18, and 25 of the ripening process. The evolution of lactic microbiota during the production and maturation of traditional cheeses involves isolating lactic acid microorganisms present in cheese. Cheese samples were analyzed for pH, fat, NaCl, fatty acids, and volatile compounds. The microbial ecosystem naturally changes during the maturation process, leading to variation in the microorganisms involved during ripening. Our results show that specific bacteria were identified in high levels during the entire ripening process and may be responsible for milk fat lipolysis contributing directly to cheese flavor by imparting detailed fatty acid flavor notes, or indirectly as precursors formation of other flavor compounds. ItemThe effect of natural antimicrobials against Campylobacter spp. and its similarities to Salmonella spp, Listeria spp., Escherichia coli, Vibrio spp., Clostridium spp. and Staphylococcus spp.(Elsevier, 2020-11-13) Balta, Igori; Linton, W. Mark R.; Pinkerton, Laurette; Kelly, Carmel A.; Stef, Lavinia; Pet, Ioan; Stef, Ducu; Criste, Adriana; Gundogdu, Ozan; Corcionivoschi, NicolaeThe increased resistance of campylobacters to antibiotics required the identification and isolation of novel antimicrobials able to inhibit its virulence, to cause less or no resistance and display no host toxicity. Acquiring all this knowledge was only possible through a better understanding of their antibacterial potency and of the biological mechanisms involved attenuating the bacterial virulence factors. This review describes the most recent developments in the area by looking at the new antimicrobial interventions aiming to combat the transmission and colonisation of Campylobacter spp. and its commonalities with other pathogenic bacteria. In this review we are also looking into the most recent developments, both in vitro and in vivo, focusing on the biological mechanisms by which natural antimicrobials express their anti-pathogenic effect. Following this extensive literature search we conclude that further studies are essential to elucidate the efficiency of plant, animal, bacteria and marine-derived antimicrobials as well as their role as promising alternatives to antibiotics. ItemIn vitro effects of phenolic acids and IgY immunoglobulins on aspects of rumen fermentation(Digital ProScholar Media (University of Bucharest), 2019) Berchez, Mihaela; Urcan, Adriana Cristina; Corcionivoschi, Nicolae; Criste, AdrianaThe current study was carried out in order to determine the effects of simple phenols and of specific anti-methanogen IgY antibodies on ruminal gas production, methane emissions, volatile fatty acids (VFA) profile and pH in in vitro ruminal cultures. Caffeic and p-Coumaric acids and IgY antibodies were added anaerobically to ruminal batch cultures. Ruminal parameters were measured after 24, 48 and 72 hours of incubation. The results showed that addition ofboth phenolic acids and IgY antibodies significantly (P <0.05) decreased methane production at 24 and 48 hours of incubation. At 24 and 48 hours of incubation some significant differences were observed in volatile fatty acids profile, while the pH was not affected by simple phenols and IgY antibodies addition. Simple phenols and IgY avian antibodies can be further tested in order to achieve the purpose of methane mitigation strategies, but the ideal way to inhibit the methanogenesis process in rumen would reduce methane production without altering the other ruminal parameters, such as VFAs, total gas production or pH. ItemNovel Insights into the Role of Probiotics in Respiratory Infections, Allergies, Cancer, and Neurological Abnormalities(MDPI, 2021-09-02) Balta, Igori; Butucel, Eugenia; Mohylyuk, Valentyn; Criste, Adriana; Dezmirean, Daniel Severus; Stef, Lavinia; Pet, Ioan; Corcionivoschi, NicolaeIn recent years, probiotics have attracted public attention and transformed the social perception of microorganisms, convening a beneficial role/state on human health. With aging, the immune system, body physiology, and intestinal microbiota tend to change unfavorably, resulting in many chronic conditions. The immune-mediated disorders can be linked to intestinal dysbiosis, consequently leading to immune dysfunctions and a cluster of conditions such as asthma, autoimmune diseases, eczema, and various allergies. Probiotic bacteria such as Lactobacillus and Bifidobacterium species are considered probiotic species that have a great immunomodulatory and anti-allergic effect. Moreover, recent scientific and clinical data illustrate that probiotics can regulate the immune system, exert anti-viral and anti-tumoral activity, and shields the host against oxidative stress. Additionally, microbiota programming by probiotic bacteria can reduce and prevent the symptoms of respiratory infections and ameliorate the neurological status in humans. This review describes the most recent clinical findings, including safe probiotic therapies aiming to medicate respiratory infections, allergies, cancer, and neurological disorders due to their physiological interconnection. Subsequently, we will describe the major biological mechanism by which probiotic bacteriotherapy expresses its anti-viral, anti-allergic, anticancer, and neuro-stimulatory effects