Genetic diversity of ruminant Pestivirus strains collected in Northern Ireland between 1999 and 2011 and the role of live ruminant imports
Date
2016-06-27
Journal Title
Journal ISSN
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Publisher
Springer Nature
Abstract
Background: The genus pestivirus within the family Flaviviridae includes bovine viral diarrhoea virus (BVDV) types 1
and 2, border disease virus (BDV) and classical swine fever virus. The two recognised genotypes of BVDV are divided
into subtypes based on phylogenetic analysis, namely a-p for BVDV-1 and a-c for BVDV-2.
Methods: Three studies were conducted to investigate the phylogenetic diversity of pestiviruses present in
Northern Ireland. Firstly, pestiviruses in 152 serum samples that had previously tested positive for BVDV between
1999 and 2008 were genotyped with a RT-PCR assay. Secondly, the genetic heterogeneity of pestiviruses from
91 serum samples collected between 2008 and 2011 was investigated by phylogenetic analysis of a 288 base
pair portion of the 5’ untranslated region (UTR). Finally, blood samples from 839 bovine and 4,437 ovine animals
imported in 2010 and 2011 were tested for pestiviral RNA. Analysis of animal movement data alongside the
phylogenetic analysis of the strains was carried out to identify any links between isolates and animal movement.
Results: No BVDV-2 strains were detected. All of the 152 samples in the first study were genotyped as BVDV-1.
Phylogenetic analysis indicated that the predominant subtype circulating was BVDV-1a (86 samples out of 91).
The remaining five samples clustered close to reference strains in subtype BVDV-1b. Out of the imported animals,
18 bovine samples tested positive and 8 inconclusive (Ct ≥36), while all ovine samples were negative. Eight
sequences were obtained and were defined as BVDV-1b. Analysis of movement data between herds failed to find
links between herds where BVDV-1b was detected.
Conclusion: Given that only BVDV-1a was detected in samples collected between 1968 and 1999, this study
suggests that at least one new subtype has been introduced to Northern Ireland between 1999 and 2011 and
highlights the potential for importation of cattle to introduce new strains.
Keywords: Bovine viral diarrhoea virus, Phylogenetic analysis, 5’untranslated region, Importation
Description
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Article
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Keywords
Bovine viral diarrhoea virus, Phylogenetic analysis, Importation, 5'untranslated region
Citation
Guelbenzu-Gonzalo, M. P., Cooper, L., Brown, C., Leinster, S., O’Neill, R., Doyle, L. and Graham, D. A. (2015) ‘Genetic diversity of ruminant Pestivirus strains collected in Northern Ireland between 1999 and 2011 and the role of live ruminant imports’, Irish Veterinary Journal. Springer Science and Business Media LLC, 69(1). doi: 10.1186/s13620-016-0066-5.