Research Note: A comparison of media for the recovery of Campylobacter spp. from long term storage at -80° C
Date
2019
Authors
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Publisher
Digital ProScholar Media (University of Bucharest)
Abstract
Pure cultures of Campylobacter spp. can be stored at -80°C for extended periods,
however they eventually lose viability. To maintain cultures regular resuscitation and subculturing
of strains needs to be undertaken but this requires that staff and media are available
for this purpose. Financial pressures on many institutes have had the consequence that regular
sub-culturing has become a financial burden.
Accordingly this study was undertaken to compare the ability of inexpensive media to
recover campylobacters from storage of up to 12 years at -80°C. Brain heart infusion agar
and modified charcoal cefoperazone deoxycholate agar base were compared with blood agar.
Overall, blood agar was found to be the best medium for this purpose and is to be
recommended.
Description
Publication history: Accepted - 23 May 2017; Published - 2019.
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Keywords
campylobacter, recovery -80°C, media, blood
Citation
Madden, C. M. and Madden, R. H. (2019) ‘Research Note: A comparison of media for the recovery of Campylobacter spp. from long term storage at -80° C’, Romanian Biotechnological Letters. Digital ProScholar Media, 24(2), pp. 340–343. doi: 10.25083/rbl/24.2/340.343.