Citation (Harvard):
Lagan Tregaskis, P., Devaney, R. and Smyth, V. J. (2021) ‘The First Whole Genome Sequence and Characterisation of Avian Nephritis Virus Genotype 3’, Viruses. MDPI AG, 13(2), p. 235. doi: 10.3390/v13020235.
Abstract:
Avian nephritis virus (ANV) is classified in the Avastroviridae family with disease associations
with nephritis, uneven flock growth and runting stunting syndrome (RSS) in chicken and turkey
flocks, and other avian species. The whole genome of ANV genotype 3 (ANV-3) of 6959 nucleotides
including the untranslated 5’ and 3’ regions and polyadenylated tail was detected in a metagenomic
virome investigation of RSS-affected chicken broiler flocks. This report characterises the ANV-3
genome, identifying partially overlapping open reading frames (ORFs), ORF1a and ORF1b, and an
opposing secondary pseudoknot prior to a ribosomal frameshift stemloop structure, with a separate
ORF2, whilst observing conserved astrovirus motifs. Phylogenetic analysis of the Avastroviridae
whole genome and ORF2 capsid polyprotein classified the first complete whole genome of ANV-3
within Avastroviridae genogroup 2.
Rights:
© 2021 by the authors.
Licensee MDPI, Basel, Switzerland.
This article is an open access article
distributed under the terms and
conditions of the Creative Commons
Attribution (CC BY) license (https://
creativecommons.org/licenses/by/
4.0/).