The First Whole Genome Sequence and Characterisation of Avian Nephritis Virus Genotype 3
Date
2021-02-03
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Publisher
MDPI
Abstract
Avian nephritis virus (ANV) is classified in the Avastroviridae family with disease associations
with nephritis, uneven flock growth and runting stunting syndrome (RSS) in chicken and turkey
flocks, and other avian species. The whole genome of ANV genotype 3 (ANV-3) of 6959 nucleotides
including the untranslated 5’ and 3’ regions and polyadenylated tail was detected in a metagenomic
virome investigation of RSS-affected chicken broiler flocks. This report characterises the ANV-3
genome, identifying partially overlapping open reading frames (ORFs), ORF1a and ORF1b, and an
opposing secondary pseudoknot prior to a ribosomal frameshift stemloop structure, with a separate
ORF2, whilst observing conserved astrovirus motifs. Phylogenetic analysis of the Avastroviridae
whole genome and ORF2 capsid polyprotein classified the first complete whole genome of ANV-3
within Avastroviridae genogroup 2.
Description
Publication history: Accepted - 27 January 2021; Published - 3 February 2021.
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Article
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Keywords
avian nephritis virus, astrovirus, phylogenetics, molecular characterisation, genotyping and recombination
Citation
Lagan Tregaskis, P., Devaney, R. and Smyth, V. J. (2021) ‘The First Whole Genome Sequence and Characterisation of Avian Nephritis Virus Genotype 3’, Viruses. MDPI AG, 13(2), p. 235. doi: 10.3390/v13020235.