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Screening method for the detection of residues of amphenicol antibiotics in bovine milk by optical biosensor

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dc.contributor.author Thompson, Colin
dc.contributor.author Traynor, Imelda
dc.contributor.author Fodey, Terence
dc.contributor.author Barnes, Paul
dc.contributor.author Faulkner, Dermot
dc.contributor.author Crooks, Steven
dc.date.accessioned 2021-05-04T11:34:07Z
dc.date.available 2021-05-04T11:34:07Z
dc.date.issued 2020-09-10
dc.identifier http://hdl.handle.net/20.500.12518/252
dc.identifier.citation Thompson, C. S., Traynor, I. M., Fodey, T. L., Barnes, P., Faulkner, D. V. and Crooks, S. R. H. (2020) ‘Screening method for the detection of residues of amphenicol antibiotics in bovine milk by optical biosensor’, Food Additives & Contaminants: Part A. Informa UK Limited, 37(11), pp. 1854–1864. doi: 10.1080/19440049.2020.1809718. en_US
dc.identifier.issn 1944-0049
dc.identifier.uri https://doi.org/10.1080/19440049.2020.1809718
dc.description Publication history: Accepted - 16 July 2020; Published online - 10 September 2020. en_US
dc.description.abstract An immunobiosensor assay was developed for multi-residue screening in bovine milk of the parent amphenicols, thiamphenicol and florfenicol, along with the metabolite florfenicol amine. A polyclonal antibody raised in a rabbit after immunisation with a florfenicol amine-protein conjugate was employed in the assay. Milk samples were subjected to acetonitrile extraction, reconstituted in buffer and diluted prior to biosensor analysis. Validation data obtained from the analysis of fortified samples has shown that the method has a detection capability of less than 0.25 μg kg−1 for florfenicol and less than 0.5 μg kg−1 for florfenicol amine and thiamphenicol. The cross-reactivity profile and validation data for the detection of these amphenicols is presented together with results obtained following the analysis of florfenicol incurred samples using the developed screening method along with a comparison of results obtained from the analysis of the same incurred samples using an MRM3 UPLC-MS/MS confirmatory method. Results are also presented obtained from the analysis of samples from both treated and non-treated animals which were co-housed and which show the potential for cross-contamination. en_US
dc.language.iso en en_US
dc.publisher Taylor & Francis en_US
dc.rights © 2020 Taylor & Francis Group, LLC. This work is made available online in accordance with the publisher’s policies. Please refer to any applicable terms of use of the publisher. en_US
dc.subject screening en_US
dc.subject detection en_US
dc.subject amphenicols en_US
dc.subject milk en_US
dc.subject optical biosensor en_US
dc.title Screening method for the detection of residues of amphenicol antibiotics in bovine milk by optical biosensor en_US
dc.type Article en_US


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