Comparison of single radial immunodiffusion and ELISA for the quantification of immunoglobulin G in bovine colostrum, milk and calf sera
Date
2017-11-02
Journal Title
Journal ISSN
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Publisher
Taylor & Francis
Abstract
The overall objective was to compare immunoglobulin G (IgG) concentrations measured by single radial
immunodiffusion (sRID) and ELISA-based methods in samples of bovine colostrum and transition milk
from contrasting breed types (Limousin × Friesian (n = 10) and Holstein (n = 10)). Jugular blood samples
were collected at 48 h post-birth from beef (n = 10) and dairy (n = 10) calves and sera harvested
subsequent to colostrum consumption. Absolute colostrum IgG values determined by ELISA showed
poor agreement with mean (SD) IgG values measured using sRID, fixed bias (sRID – ELISA) was 31.89
(±9.84) mg/mL; having wide limits of agreement (12.61–51.17) and a low concordance coefficient
(0.26). The agreement between ELISA and sRID when measuring serum IgG was greater than that of
colostrum, fixed bias (sRID – ELISA) was 12.36 (±6.60) mg/mL; having narrower limits of agreement
(−0.58 to 25.30) and serum IgG concentrations had a greater concordance coefficient (0.44) between
samples. Calf sera IgG measured using the indirect zinc sulphate turbidity test showed a strong
correlation with the sRID and ELISA methods (P < .001), R2 = 0.78 and R2 = 0.77 respectively. Overall, the
ELISA and sRID methodologies had a strong positive association with almost a twofold (1.8) difference
between values; therefore, they provide diverse absolute values of IgG concentration.
Description
Publication history: Accepted - 12 October 2017; Published online - 02 November 2017.
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Article
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Keywords
ELISA, single radial immunodiffusion, zinc sulphate turbidity test, Brix refractometer, IgG, calf, colostrum
Citation
Dunn, A., Duffy, C., Gordon, A., Morrison, S., Argűello, A., Welsh, M. and Earley, B. (2017) ‘Comparison of single radial immunodiffusion and ELISA for the quantification of immunoglobulin G in bovine colostrum, milk and calf sera’, Journal of Applied Animal Research, 46(1), pp. 758–765. doi: 10.1080/09712119.2017.1394860.