A Novel Natural Antimicrobial Can Reduce the in vitro and in vivo Pathogenicity of T6SS Positive Campylobacter jejuni and Campylobacter coli Chicken Isolates
Date
2018-09-07
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Publisher
Frontiers Media
Abstract
Human campylobacteriosis is considered one of the most common foodborne diseases
worldwide with poultry identified as the main source of infection accounting for 50–
80% of human cases. Highly virulent Campylobacter spp., positive for the Type VI
secretion system (T6SS), which have an increased ability to adhere to and invade
the host gastrointestinal epithelium are highly prevalent in poultry. Multidrug resistant
strains of bacteria are rapidly evolving and therefore, new antimicrobials to supplement
animal feed that are able to control Campylobacter species, are in great need. The
work presented herein indicates that a novel phenolic antimicrobial, Auranta 3001, is
able to reduce the adhesion and invasion of human intestinal epithelial cells (HCT-
8) by two T6SS positive chicken isolates, C. jejuni RC039 (p < 0.05) and C. coli
RC013 (p < 0.001). Exposure of C. jejuni RC039 and C. coli RC013 to Auranta 3001
downregulated the expression of hcp and cetB genes, known to be important in the
functionality of T6SS. Furthermore, the reduced adhesion and invasion is associated
with a significant decrease in bacterial motility of both isolates (p < 0.05–p < 0.001)
in vitro. Most importantly our in vivo results show that Auranta 3001 is able to reduce
cecum colonization levels from log 8 CFU/ml to log 2 CFU/ml for C. jejuni RC039
and from log 7 CFU/ml to log 2 CFU/ml for C. coli RC013. In conclusion, this novel
antimicrobial is able to reduce the pathogenic properties of T6SS campylobacters in vitro
and also to decrease colonization in vivo.
Description
Publication history: Accepted - 21 August 2018; Published - 07 September 2018.
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Article
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Keywords
Campylobacter jejuni, Campylobacter coli, HCT-8, attachment, invasion, gene expression
Citation
Sima, F., Stratakos, A. Ch., Ward, P., Linton, M., Kelly, C., Pinkerton, L., Stef, L., Gundogdu, O., Lazar, V. and Corcionivoschi, N. (2018) ‘A Novel Natural Antimicrobial Can Reduce the in vitro and in vivo Pathogenicity of T6SS Positive Campylobacter jejuni and Campylobacter coli Chicken Isolates’, Frontiers in Microbiology, 9. doi: 10.3389/fmicb.2018.02139.