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Diagnosis of sheep fasciolosis caused by Fasciola hepatica using cathepsin L enzyme-linked immunosorbent assays (ELISA)

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dc.contributor.author Lopez Corrales, Jesús
dc.contributor.author Cwiklinski, Krystyna
dc.contributor.author De Marco Verissimo, Carolina
dc.contributor.author Dorey, Amber
dc.contributor.author Lalor, Richard
dc.contributor.author Jewhurst, Heather
dc.contributor.author McEvoy, Amanda
dc.contributor.author Diskin, Michael
dc.contributor.author Duffy, Catherine
dc.contributor.author Cosby, S. Louise
dc.contributor.author Keane, Orla M.
dc.contributor.author Dalton, John Pius
dc.date.accessioned 2021-09-17T15:00:02Z
dc.date.available 2021-09-17T15:00:02Z
dc.date.issued 2021-07-06
dc.identifier http://hdl.handle.net/20.500.12518/352
dc.identifier.citation López Corrales, J., Cwiklinski, K., De Marco Verissimo, C., Dorey, A., Lalor, R., Jewhurst, H., McEvoy, A., Diskin, M., Duffy, C., Cosby, S. L., Keane, O. M. and Dalton, J. P. (2021) ‘Diagnosis of sheep fasciolosis caused by Fasciola hepatica using cathepsin L enzyme-linked immunosorbent assays (ELISA)’, Veterinary Parasitology, 298, p. 109517. doi: 10.1016/j.vetpar.2021.109517. en_US
dc.identifier.issn 0304-4017
dc.identifier.uri https://doi.org/10.1016/j.vetpar.2021.109517
dc.description Publication history: Accepted - 3 July 2021; Published online - 6 July 2021. en_US
dc.description.abstract Fasciolosis, a global parasitic disease of agricultural livestock, is caused by the liver fluke Fasciola hepatica. Management and strategic control of fasciolosis on farms depends on early assessment of the extent of disease so that control measures can be implemented quickly. Traditionally, this has relied on the detection of eggs in the faeces of animals, a laborious method that lacks sensitivity, especially for sub-clinical infections, and identifies chronic infections only. Enzyme linked immunosorbent assays (ELISA) offer a quicker and more sensitive serological means of diagnosis that could detect early acute infection before significant liver damage occurs. The performance of three functionally-active recombinant forms of the major F. hepatica secreted cathepsins L, rFhCL1, rFhCL2, rFhCL3, and a cathepsin B, rFhCB3, were evaluated as antigens in an indirect ELISA to serologically diagnose liver fluke infection in experimentally and naturally infected sheep. rFhCL1 and rFhCL3 were the most effective of the four antigens detecting fasciolosis in sheep as early as three weeks after experimental infection, at least five weeks earlier than both coproantigen and faecal egg tests. In addition, the rFhCL1 and rFhCL3 ELISAs had a very low detection limit for liver fluke in lambs exposed to natural infection on pastures and thus could play a major role in the surveillance of farms and a ‘test and treat’ approach to disease management. Finally, antibodies to all three cathepsin L proteases remain high throughout chronic infection but decline rapidly after drug treatment with the flukicide, triclabendazole, implying that the test may be adapted to trace the effectiveness of drug treatment. en_US
dc.description.sponsorship This work was supported by a European Research Council Advanced Grant (HELIVAC, 322725) and Science Foundation Ireland (SFI) Professorship grant (17/RP/5368) awarded to J.P. Dalton. en_US
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.rights © 2021 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). en_US
dc.subject Fasciola hepatica en_US
dc.subject Diagnostics en_US
dc.subject ELISA en_US
dc.subject Coproantigen en_US
dc.subject Cathepsin L peptidases en_US
dc.title Diagnosis of sheep fasciolosis caused by Fasciola hepatica using cathepsin L enzyme-linked immunosorbent assays (ELISA) en_US
dc.type Article en_US


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