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Associations between Circulating IGF-1 Concentrations, Disease Status and the Leukocyte Transcriptome in Early Lactation Dairy Cows

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dc.contributor.author Wathes, D. Claire
dc.contributor.author Becker, Frank
dc.contributor.author Buggiotti, Laura
dc.contributor.author Crowe, Mark A.
dc.contributor.author Ferris, Conrad
dc.contributor.author Foldager, Leslie
dc.contributor.author Grelet, Clément
dc.contributor.author Hostens, Miel
dc.contributor.author Ingvartsen, Klaus L.
dc.contributor.author Marchitelli, Cinzia
dc.contributor.author Salavati, Mazdak
dc.contributor.author Signorelli, Federica
dc.contributor.author Takeda, Haruko
dc.contributor.author Tang, Lijing
dc.contributor.author Cheng, Zhangrui
dc.contributor.author GplusE Consortium
dc.date.accessioned 2022-03-28T12:09:28Z
dc.date.available 2022-03-28T12:09:28Z
dc.date.issued 2021-11-25
dc.identifier http://hdl.handle.net/20.500.12518/422
dc.identifier.citation Wathes, D.C., Becker, F., Buggiotti, L., Crowe, M.A., Ferris, C., Foldager, L., Grelet, C., Hostens, M., Ingvartsen, K.L., Marchitelli, C., Salavati, M., Signorelli, F., Takeda, H., Tang, L. and Cheng, Z. (2021) ‘Associations between Circulating IGF-1 Concentrations, Disease Status and the Leukocyte Transcriptome in Early Lactation Dairy Cows’, Ruminants. MDPI AG. doi:10.3390/ruminants1020012. en_US
dc.identifier.issn 2673-933X (electronic)
dc.identifier.uri https://doi.org/10.3390/ruminants1020012
dc.description Publication history: Accepted - 19 November 2021; Published - 25 November 2021. en_US
dc.description.abstract Peripartum dairy cows commonly experience negative energy balance (EB) and immunosuppression together with high incidences of infectious and metabolic disease. This study investigated mechanisms linking EB status with immune defense in early lactation. Data were collected from multiparous Holstein cows from six herds and leukocyte transcriptomes were analyzed using RNA sequencing. Global gene expression was related to circulating IGF-1 (as a biomarker for EB) by subdividing animals into three groups, defined as IGF-1 LOW (<35 ng/mL, n = 35), MODERATE (35–100 ng/mL, n = 92) or HIGH (>100 ng/mL, n = 43) at 14 ± 4 days in milk (DIM). Differentially expressed genes between groups were identified using CLC Genomics Workbench V21, followed by cluster and KEGG pathway analysis, focusing on the comparison between LOW and HIGH IGF-1 cows. LOW cows were older and had significantly lower dry matter intakes and EB values, whereas HIGH cows produced more milk. During the first 35 DIM, 63% of LOW cows had more than one health problem vs. 26% HIGH cows, including more with clinical mastitis and uterine infections. Gene expression analysis indicated that leukocytes in LOW cows switched energy metabolism from oxidative phosphorylation to aerobic glycolysis (PGM, LDH, and PDK4). Many antimicrobial peptides were up-regulated in LOW cows (e.g., PTX3, DMBT1, S100A8, and S100A9) together with genes associated with inflammation, platelet activation and the complement cascade. HIGH cows had greater expression of genes regulating T and B cell function and the cytoskeleton. Overall, results suggested an ongoing cycle of poor EB and higher infection rates in LOW IGF-1 cows which was reflected in altered leukocyte functionality and reduced milk production. en_US
dc.description.sponsorship This project received funding from the European Union’s Seventh Framework Programme (Brussels, Belgium) for research, technological development, and demonstration under grant agreement no. 613689 en_US
dc.language.iso en en_US
dc.publisher MDPI en_US
dc.rights Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). en_US
dc.subject IGF-1 en_US
dc.subject leukocyte en_US
dc.subject energy balance en_US
dc.subject RNA-seq en_US
dc.subject dairy cow en_US
dc.subject antimicrobial en_US
dc.subject inflammation en_US
dc.subject mastitis en_US
dc.subject PTX3 en_US
dc.subject DMBT1 en_US
dc.title Associations between Circulating IGF-1 Concentrations, Disease Status and the Leukocyte Transcriptome in Early Lactation Dairy Cows en_US
dc.type Article en_US

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