The Expression of Terpenoid Indole Alkaloid (TIAs) Pathway Genes in Catharanthus roseus in Response to Salicylic Acid treatment

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dc.contributor.authorSoltani, Narges
dc.contributor.authorNazarian-Firouzabadi, Farhad
dc.contributor.authorShafeinia, Alireza
dc.contributor.authorSadr, Ayeh Sadat
dc.contributor.authorShirali, Masoud
dc.date.accessioned2020-08-26T13:15:18Z
dc.date.available2020-08-26T13:15:18Z
dc.date.issued2020-08-26
dc.descriptionPublication history: Accepted - 28th August 2020; Published online - 4th September.
dc.description.abstractVinblastine and vincristine are two important anti-cancer drugs that are synthesized by the Terpenoid Indole Alkaloids (TIAs) pathway in periwinkle (Catharanthus roseus). The major challenge in the pharmaceutical industry is the low production rate of these alkaloids. TIA pathway is affected by elicitors, such as salicylic acid (SA). This study aimed to investigate the expression pattern of some key genes in TIAs pathway under SA treatment. Foliar application of SA (0.01 and 0.1 mM) was used and leaves samples were taken at 0, 12, 18, 24 and 48 hours after the treatment. qRT-PCR was used to investigate the expression pattern of Chorismate mutase (Cm), Tryptophan decarboxylase (Tdc), Geraniol-10-hydroxylase (G10h), Secologanin synthase (Sls), Strictosidine synthase (Str), Desacetoxyvindoline-4-hydroxylase (D4h) and Deacetylvindoline-4-O-acetyltransferase (Dat) genes, following the SA treatment. The results of this experiment showed that transcript levels of Tdc, G10h, Sls, Str, D4h and Dat genes were significantly up-regulated in both SA concentration treatments. Furthermore, the highest transcript levels of Dat was observed after 48 hours of the SA treatments. qRT-PCR results suggests that SA induces transcription of major genes involved in alkaloids biosynthesis in Catharanthus roseus. It can be concluded that up-regulation of Tdc, G10h, Sls, Str, D4h and Dat genes can result in a higher production rate of vinblastine and vincristine alkaloids.en_US
dc.identifierhttp://hdl.handle.net/20.500.12518/169
dc.identifier.citationSoltani, N., Nazarian-Firouzabadi, F., Shafeinia, A., Sadr, A.S. and Shirali, M. (2020) ‘The expression of Terpenoid Indole Alkaloid (TIAs) pathway genes in Catharanthus roseus in response to salicylic acid treatment’, Molecular Biology Reports. Springer Science and Business Media LLC. doi:10.1007/s11033-020-05759-y
dc.identifier.issn1573-4978
dc.identifier.urihttps://doi.org/10.1007/s11033-020-05759-y
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.rights© Springer Nature B.V. 2020. This work is made available online in accordance with the publisher’s policies. Please refer to any applicable terms of use of the publisher.
dc.subjectElicitor, Gene Expression, Salicylic Acid, Terpenoid Indole Alkaloids, TIAs pathway, qRT-PCRen_US
dc.titleThe Expression of Terpenoid Indole Alkaloid (TIAs) Pathway Genes in Catharanthus roseus in Response to Salicylic Acid treatmenten_US
dc.typeArticleen_US

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