Bioinformatics analysis of differentially gene expression profiles related to heat stress in brain, liver, and leg muscle of broiler chickens based on microarray technique

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dc.contributor.authorEkhtiyari, Mohammad Soleiman
dc.contributor.authorJavanmard, Arash
dc.contributor.authorGhafouri, Farzad
dc.contributor.authorSadr, Ayeh Sadat
dc.contributor.authorMiraei-Ashtiani, Seyed Reza
dc.contributor.authorShirali, Masoud
dc.contributor.departmentEconomics
dc.date.accessioned2023-10-16T14:33:47Z
dc.date.available2023-10-16T14:33:47Z
dc.date.issued2023-09-23
dc.descriptionPublication history: Accepted - 5 August 2022; Published online - 23 September 2023.
dc.description.abstractIn the poultry industry, the heat stress caused by high environmental temperature has a negative influence on broiler chicken performance and has become a major challenge. Transcriptome profile analysis of the data and identification of patterns of differential gene expression in related tissues can be involved in the discovery of molecular mechanisms resistant to heat stress. The main purpose of this study was to use transcriptome profiles of three tissues brain, liver, and leg muscle of two groups of the control and heat stress broiler chickens to identify candidate genes associated with heat stress. By the analysis of microarray data to express the gene differences, 657 significant genes (P<0.05) were extracted, which a total of 94 genes showed significant expression differences (FDR < 0.05, Fold change > ± 2). Then, by studying the ontology of the relevant genes resulting from data analysis and literature mining as well as the reconstructed protein-protein interaction network, hub genes including NSDHL, DHCR24, LSS, FDPS, PCK1, ACTA1, HSP90AA1, HSPA2, HSPB1, HSF1, CRYAB, APOB, and IL6 were identified. Annotation results of these genes indicated that they have a role in the main process of metabolic and signaling pathways related to the ion transport system, steroid, antibodies, cholesterol biosynthesis, lipid metabolism, immune system function, and various signaling pathways such as MAP kinase, RET, and ERK. Overall, the present study can provide new insights into evidence of the pathways activated by these genes to identify effective genes and a better understanding of biological processes related to heat stress.
dc.identifierhttps://hdl.handle.net/20.500.12518/589
dc.identifier.citationEkhtiyari, M. S., Javanmard, A., Ghafouri, F., Sadat Sadr, A., Miraei-Ashtiani, S. R., Shirali, M. (2023). 'Bioinformatics analysis of differentially gene expression profiles related to heat stress in brain, liver, and leg muscle of broiler chickens based on microarray technique', Iranian Journal of animal Science, 54(3), pp. 203-223. doi: 10.22059/ijas.2022.334755.653860
dc.identifier.issn2008-4773
dc.identifier.urihttps://doi.org/10.22059/ijas.2022.334755.653860
dc.language.isoother
dc.publisherUniversity of Tehran Press
dc.rights© The Author(s). CC-BY-NC license: https://creativecommons.org/licenses/by-nc/4.0/
dc.subjectBroiler chickens
dc.subjectGene expression
dc.subjectHeat stress
dc.subjectHub genes
dc.subjectSystem biology
dc.titleBioinformatics analysis of differentially gene expression profiles related to heat stress in brain, liver, and leg muscle of broiler chickens based on microarray technique
dc.typeArticle
dcterms.dateAccepted2022-08-05
dcterms.dateSubmitted2021-11-30
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