Effect of different levels of protein concentrates supplementation on the growth performance, plasma amino acids profile and mTOR cascade genes expression in early-weaned yak calves

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dc.contributor.authorPeng, Q.H.
dc.contributor.authorKhan, N.A.
dc.contributor.authorXue, B.
dc.contributor.authorYan, Tianhai
dc.contributor.authorWang, Z.S.
dc.date.accessioned2021-06-23T10:48:17Z
dc.date.available2021-06-23T10:48:17Z
dc.date.issued2017-06-27
dc.descriptionPublication history: Accepted - 13 June 2017; Published online - 27 June 2017en_US
dc.description.abstractObjective This study evaluated the effects of different levels of protein concentrate supplementation on the growth performance of yak calves, and correlated the growth rate to changes occurring in the plasma- amino acids, -insulin profile, and signaling activity of mammalian target of rapamycin (mTOR) cascade to characterize the mechanism through which the protein synthesis can be improved in early weaned yaks. Methods For this study, 48 early (3 months old) weaned yak calves were selected, and assigned into four dietary treatments according to randomized complete block design. The four blocks were balanced for body weight and sex. The yaks were either grazed on natural pasture (control diet) in a single herd or the grazing yaks was supplemented with one of the three protein rich supplements containing low (17%; LP), medium (19%; MP), or high (21%; HP) levels of crude proteins for a period of 30 days. Results Results showed that the average daily gain of calves increased (0.14 vs 0.23–0.26 kg; p<0.05) with protein concentrates supplementation. The concentration of plasma methionine increased (p<0.05; 8.6 vs 10.1–12.4 μmol/L), while those of serine and tyrosine did not change (p>0.05) when the grazing calves were supplemented with protein concentrates. Compared to control diet, the insulin level of calves increased (p<0.05; 1.86 vs 2.16–2.54 μIU/mL) with supplementation of protein concentrates. Addition of protein concentrates up-regulated (p<0.05) expression of mTOR-raptor, mammalian vacuolar protein sorting 34 homolog, the translational regulators eukaryotic translation initiation factor 4E binding protein 1, and S6 kinase 1 genes in both Longissimus dorsi and semitendinosus. In contrast, the expression of sequestosome 1 was down-regulated in the concentrate supplemented calves. Conclusion Our results show that protein supplementation improves the growth performance of early weaned yak calves, and that plasma methionine and insulin concentrations were the key mediator for gene expression and protein deposition in the muscles.en_US
dc.description.sponsorshipThis research project was supported by grants from the Cattle System Special Research Funds (CARS-38).en_US
dc.identifierhttp://hdl.handle.net/20.500.12518/295
dc.identifier.citationPeng, Q. H., Khan, N. A., Xue, B., Yan, T. H. and Wang, Z. S. (2018) ‘Effect of different levels of protein concentrates supplementation on the growth performance, plasma amino acids profile and mTOR cascade genes expression in early-weaned yak calves’, Asian-Australasian Journal of Animal Sciences, 31(2), pp. 218–224. doi: 10.5713/ajas.16.0999.en_US
dc.identifier.issn2765-0189
dc.identifier.issn2765-0235
dc.identifier.urihttps://doi.org/10.5713/ajas.16.0999
dc.language.isoenen_US
dc.publisherAsian-Australasian Association of Animal Production Societies (AAAP)en_US
dc.rightsCopyright © 2018 by Asian-Australasian Journal of Animal Sciences This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.subjectEarly Weaningen_US
dc.subjectSupplementary Feedingen_US
dc.subjectProtein Synthesisen_US
dc.subjectYaken_US
dc.subjectYak Calves Growthen_US
dc.titleEffect of different levels of protein concentrates supplementation on the growth performance, plasma amino acids profile and mTOR cascade genes expression in early-weaned yak calvesen_US
dc.typeArticleen_US
dcterms.dateAccepted2017-06-13
dcterms.dateSubmitted2016-12-10

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