Messenger RNA biomarkers of Bovine Respiratory Syncytial Virus infection in the whole blood of dairy calves

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dc.contributor.authorJohnston, Dayle
dc.contributor.authorEarley, Bernadette
dc.contributor.authorMcCabe, Matthew S.
dc.contributor.authorKim, JaeWoo
dc.contributor.authorTaylor, Jeremy F.
dc.contributor.authorLemon, Ken
dc.contributor.authorDuffy, Catherine
dc.contributor.authorMcMenamy, Michael
dc.contributor.authorCosby, S. Louise
dc.contributor.authorWaters, Sinead M.
dc.contributor.departmentAnimal Health and Welfare
dc.date.accessioned2024-03-25T11:14:42Z
dc.date.available2024-03-25T11:14:42Z
dc.date.issued2021-04-30
dc.descriptionPublication history: Accepted - 30 March 2021; Published online - 30 April 2021
dc.description.abstractBovine Respiratory Syncytial Virus (BRSV) is a primary viral cause of Bovine Respiratory Disease (BRD) in young calves, which is responsible for substantial morbidity and mortality. Infection with BRSV induces global gene expression changes in respiratory tissues. If these changes are observed in tissues which are more accessible in live animals, such as whole blood, they may be used as biomarkers for diagnosis of the disease. Therefore, the objective of the current study was to elucidate the whole blood transcriptomic response of dairy calves to an experimental challenge with BRSV. Calves (Holstein–Friesian) were either administered BRSV inoculate (103.5 TCID50/ml × 15 ml) (n = 12) or sterile phosphate buffered saline (n = 6). Clinical signs were scored daily and whole blood was collected in Tempus RNA tubes immediately prior to euthanasia, at day 7 post-challenge. RNA was extracted from blood and sequenced (150 bp paired-end). The sequence reads were aligned to the bovine reference genome (UMD3.1) and EdgeR was subsequently employed for differential gene expression analysis. Multidimensional scaling showed that samples from BRSV challenged and control calves segregated based on whole blood gene expression changes, despite the BRSV challenged calves only displaying mild clinical symptoms of the disease. There were 281 differentially expressed (DE) genes (p < 0.05, FDR < 0.1, fold change > 2) between the BRSV challenged and control calves. The top enriched KEGG pathways and gene ontology terms were associated with viral infection and included “Influenza A”, “defense response to virus”, “regulation of viral life cycle” and “innate immune response”. Highly DE genes involved in these pathways may be beneficial for the diagnosis of subclinical BRD from blood samples.
dc.description.sponsorshipThis project was funded by the Irish Department of Agriculture and the Department of Agriculture, Environment and Rural Affairs, Northern Ireland, as part of the US-Ireland R&D partnership call (RMIS_0033 Project 16/RD/US-ROI/11). JFT and JWK were supported by Grant No. 2017-67015-26760 from the United States Department for Agriculture National Institute for Food and Agriculture.
dc.identifierhttps://hdl.handle.net/20.500.12518/693
dc.identifier.citationJohnston, D., Earley, B., McCabe, M.S., Kim, J., Taylor, J.F., Lemon, K., Duffy, C., McMenamy, M., Cosby, S.L. and Waters, S.M. (2021) ‘Messenger RNA biomarkers of Bovine Respiratory Syncytial Virus infection in the whole blood of dairy calves’, Scientific Reports. Springer Science and Business Media LLC. Available at: https://doi.org/10.1038/s41598-021-88878-1.
dc.identifier.issn2045-2322
dc.identifier.urihttps://doi.org/10.1038/s41598-021-88878-1
dc.language.isoen
dc.publisherNature Research
dc.rightsOpen Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
dc.titleMessenger RNA biomarkers of Bovine Respiratory Syncytial Virus infection in the whole blood of dairy calves
dc.typeArticle
dcterms.dateAccepted2021-03-30
dcterms.dateSubmitted2020-11-04

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