Evaluation of thermogravimetric analysis as a rapid tool for the detection of rhizobacteria biostimulants used in precision agriculture

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dc.contributor.authorCarmichael, Eugene
dc.contributor.authorRao, Juluri R.
dc.date.accessioned2022-02-23T14:53:48Z
dc.date.available2022-02-23T14:53:48Z
dc.date.issued2021-01-17
dc.descriptionPublication history: Accepted - 31 October 2020; Published online - 17 January 2021en_US
dc.description.abstractSix-week-old root samples were evaluated using high resolution (maximum resolution [MaxRes]) thermogravimetric analyses (TGA) of the cell wall compositions of Gram-positive (Bacillus mucilaginosus, Bacillus amyloliquefaciens, and Bacillus subtilis) and Gram-negative (Burkholderia sp., Rahnella aquatilis strain H 2.6, and R. aquatilis strain RC 2.5) root colonizing plant growth promoting rhizobacteria (PGPR) commercial inoculant strains (biostimulants) applied to pot grown wheat plants. TGA discriminated the strains within the two types of rhizobacterial cohorts and thus provided a rapid non-molecular means for the detection of PGPR inoculant biostimulants within hours of root sampling. The latter was due to the greater degree of definition of TGA fingerprints of individual thermal weight loss events occurring over a degradation range, and heightened the corresponding peak temperature divergences within strains of either type of bacteria themselves for their unequivocal identification. Confirmation of biostimulant rhizobacteria identity in concomitant root samples was achieved through either cultural methods or direct tissue PCR molecular protocols within 5 days and 2 days of sampling, respectively. The results suggested that MaxRes TGA could serve as a rapid, inexpensive stand-alone tool or as combinatorial utility alongside pyrolysis gas chromatography mass spectra, and Fourier transform infrared analytics for the early detection of PGPR biostimulants in precision farmingen_US
dc.description.sponsorshipWe thank EU-BIOFECTOR (Grant Agreement No. 312117) funded by the European Commission within the 7th Framework Programme, and the Department of Agriculture, Environment and Rural Affairs (DAERA), Northern Ireland, UK for their Evidence and Innovation grant (activity 48125) to carry out the study at the Agri-Food & Biosciences Institute laboratories (AFBINI.GOV.UK)en_US
dc.identifierhttp://hdl.handle.net/20.500.12518/408
dc.identifier.citationCarmichael, E. and Rao, J.R. (2021) ‘Evaluation of thermogravimetric analysis as a rapid tool for the detection of rhizobacteria biostimulants used in precision agriculture’, Journal of Applied Biology & Biotechnology. Journal of Applied Pharmaceutical Science. doi:10.7324/jabb.2021.9102en_US
dc.identifier.issn2347-212X
dc.identifier.issn2455-7005
dc.identifier.urihttps://doi.org/10.7324/JABB.2021.9102
dc.language.isoenen_US
dc.publisherOpen Science Publishers LLPen_US
dc.rights© 2021 Carmichael and Rao. This is an open access article distributed under the terms of the Creative Commons Attribution License -NonCommercial-ShareAlike Unported License (http://creativecommons.org/licenses/by-nc-sa/3.0/).en_US
dc.subjectThermogravimetryen_US
dc.subjectFourier Transform Infra-Red (FTIR)en_US
dc.subjectSoil and plant rhizosphereen_US
dc.subjectrhizobacteriaen_US
dc.subjectthermal degradationen_US
dc.titleEvaluation of thermogravimetric analysis as a rapid tool for the detection of rhizobacteria biostimulants used in precision agricultureen_US
dc.typeArticleen_US
dcterms.dateAccepted2020-10-31
dcterms.dateSubmitted2020-09-22

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