MRM3-based UHPLC-MS/MS method for quantitation of total florfenicol residue content in milk and withdrawal study of profile of milk from treated cows

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dc.contributor.authorFaulkner, Dermot V.
dc.contributor.authorCantley, Lynne M.
dc.contributor.authorKennedy, David G.
dc.contributor.authorElliott, Christopher T.
dc.contributor.authorCrooks, Steven
dc.date.accessioned2022-04-21T11:31:47Z
dc.date.available2022-04-21T11:31:47Z
dc.date.issued2022-01-06
dc.descriptionPublication history: Accepted - 3 Jan 2022; Published online - 6 Jan 2022en_US
dc.description.abstractFlorfenicol is a broad spectrum antibacterial, licensed globally for treatment of animal and aquaculture diseases. In the EU, Canada and US it is not permitted for use in animals producing milk or eggs. There are no published methods for analysis of total florfenicol content in milk/milk products as these lack a hydrolysis step, failing to meet the marker residue definition. A method for determining total florfenicol content in milk that meets this definition is reported for the first time. Use of a UHPLC-MS/MS multiple reaction monitoring-cubed method improved the selective detection and quantitation of lower levels of florfenicol amine in milk compared to MRM only. Single laboratory validation data and withdrawal profile in bovine milk are presented. A withdrawal period of over 50 days is indicated in case of off-label use. Requirement for hydrolysis is demonstrated.en_US
dc.identifierhttp://hdl.handle.net/20.500.12518/428
dc.identifier.citationFaulkner, D.V., Cantley, M.L., Kennedy, D.G., Elliott, C.T. and Crooks, S.R.H. (2022) ‘MRM3-based UHPLC-MS/MS method for quantitation of total florfenicol residue content in milk and withdrawal study profile of milk from treated cows’, Food Chemistry. Elsevier BV. doi:10.1016/j.foodchem.2022.132070.en_US
dc.identifier.issn0308-8146
dc.identifier.urihttps://doi.org/10.1016/j.foodchem.2022.132070
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rightsCopyright 2022 Elsevier. This manuscript is distributed under a Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits distribution and reproduction for non-commercial purposes, provided the author and source are cited.en_US
dc.subjectFlorfenicol amineen_US
dc.subjectMilken_US
dc.subjectHydrolysisen_US
dc.subjectWithdrawal studyen_US
dc.subjectLC-MS/MS-MRM3en_US
dc.subjectvALIDATIONen_US
dc.titleMRM3-based UHPLC-MS/MS method for quantitation of total florfenicol residue content in milk and withdrawal study of profile of milk from treated cowsen_US
dc.typeArticleen_US
dcterms.dateAccepted2022-01-03
dcterms.dateSubmitted2021-04-12

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