RAD sequencing resolves fine-scale population structure in a benthic invertebrate: implications for understanding phenotypic plasticity

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dc.contributor.authorVendrami, David
dc.contributor.authorTelesca, Luca
dc.contributor.authorWeigand, Hannah
dc.contributor.authorWeiss, Martina
dc.contributor.authorFawcett, Katie
dc.contributor.authorLehman, Katrin
dc.contributor.authorClark, M.S.
dc.contributor.authorLeese, Florian
dc.contributor.authorMcMinn, Carrie
dc.contributor.authorMoore, Heather M.
dc.contributor.authorHoffman, Joseph Il
dc.date.accessioned2021-07-14T15:11:39Z
dc.date.available2021-07-14T15:11:39Z
dc.date.issued2017-02-01
dc.descriptionPublication history: Accepted - 4 January 2017; Published online - 1 February 2017.en_US
dc.description.abstractThe field of molecular ecology is transitioning from the use of small panels of classical genetic markers such as microsatellites to much larger panels of single nucleotide polymorphisms (SNPs) generated by approaches like RAD sequencing. However, few empirical studies have directly compared the ability of these methods to resolve population structure. This could have implications for understanding phenotypic plasticity, as many previous studies of natural populations may have lacked the power to detect genetic differences, especially over micro-geographic scales. We therefore compared the ability of microsatellites and RAD sequencing to resolve fine-scale population structure in a commercially important benthic invertebrate by genotyping great scallops (Pecten maximus) from nine populations around Northern Ireland at 13 microsatellites and 10 539 SNPs. The shells were then subjected to morphometric and colour analysis in order to compare patterns of phenotypic and genetic variation. We found that RAD sequencing was superior at resolving population structure, yielding higher Fst values and support for two distinct genetic clusters, whereas only one cluster could be detected in a Bayesian analysis of the microsatellite dataset. Furthermore, appreciable phenotypic variation was observed in size-independent shell shape and coloration, including among localities that could not be distinguished from one another genetically, providing support for the notion that these traits are phenotypically plastic. Taken together, our results suggest that RAD sequencing is a powerful approach for studying population structure and phenotypic plasticity in natural populations.en_US
dc.identifierhttp://hdl.handle.net/20.500.12518/310
dc.identifier.citationVendrami, D. L. J., Telesca, L., Weigand, H., Weiss, M., Fawcett, K., Lehman, K., Clark, M. S., Leese, F., McMinn, C., Moore, H. and Hoffman, J. I. (2017) ‘RAD sequencing resolves fine-scale population structure in a benthic invertebrate: implications for understanding phenotypic plasticity’, Royal Society Open Science, 4(2), p. 160548. doi: 10.1098/rsos.160548.en_US
dc.identifier.issn2054-5703
dc.identifier.urihttps://doi.org/10.1098/rsos.160548
dc.language.isoenen_US
dc.publisherRoyal Society Publishingen_US
dc.rights© 2017 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. Creative Commons Attribution Licenseen_US
dc.subjectPecten maximusen_US
dc.subjectgreat scallopen_US
dc.subjectphenotypic plasticityen_US
dc.subjectsimple nucleotide polymorphismen_US
dc.subjectmicrosatelliteen_US
dc.subjectmorphometricsen_US
dc.titleRAD sequencing resolves fine-scale population structure in a benthic invertebrate: implications for understanding phenotypic plasticityen_US
dc.typeArticleen_US
dcterms.dateAccepted2017-01-04
dcterms.dateSubmitted2016-07-26

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