Cytochrome P450 168A1 from Pseudomonas aeruginosa is involved in the hydroxylation of biologically relevant fatty acids

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dc.contributor.authorPrice, Claire L.
dc.contributor.authorWarrilow, Andrew G. S.
dc.contributor.authorRolley, Nicola J.
dc.contributor.authorParker, Josie E.
dc.contributor.authorThoss, Vera
dc.contributor.authorKelly, Diane E.
dc.contributor.authorCorcionivoschi, Nicolae
dc.contributor.authorKelly, Steven L.
dc.date.accessioned2022-05-31T15:10:16Z
dc.date.available2022-05-31T15:10:16Z
dc.date.issued2022-03-21
dc.descriptionPublication history: Accepted - 24 February 2022; Published - 21 March 2022.en_US
dc.description.abstractThe cytochrome P450 CYP168A1 from Pseudomonas aeruginosa was cloned and expressed in Escherichia coli followed by purification and characterization of function. CYP168A1 is a fatty acid hydroxylase that hydroxylates saturated fatty acids, including myristic (0.30 min-1), palmitic (1.61 min-1) and stearic acids (1.24 min-1), at both the ω-1- and ω-2-positions. However, CYP168A1 only hydroxylates unsaturated fatty acids, including palmitoleic (0.38 min-1), oleic (1.28 min-1) and linoleic acids (0.35 min-1), at the ω-1-position. CYP168A1 exhibited a catalytic preference for palmitic, oleic and stearic acids as substrates in keeping with the phosphatidylcholine-rich environment deep in the lung that is colonized by P. aeruginosa.en_US
dc.description.sponsorshipThis work was supported by the European Union European Regional Development Fund (ERDF) via the Wales European Funding Office of the Welsh Government through the BEACON funding initiative.en_US
dc.identifierhttp://hdl.handle.net/20.500.12518/441
dc.identifier.citationPrice CL, Warrilow AGS, Rolley NJ, Parker JE, Thoss V, Kelly DE, et al. (2022), 'Cytochrome P450 168A1 from Pseudomonas aeruginosa is involved in the hydroxylation of biologically relevant fatty acids', PLoS One. PLoS. doi:10.1371/journal.pone.0265227en_US
dc.identifier.issn1932-6203 (electronic)
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0265227
dc.language.isoenen_US
dc.publisherPLoS (Public Library of Science)en_US
dc.rights© 2022 Price et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.titleCytochrome P450 168A1 from Pseudomonas aeruginosa is involved in the hydroxylation of biologically relevant fatty acidsen_US
dc.typeArticleen_US
dcterms.dateAccepted2022-02-24
dcterms.dateSubmitted2021-09-11

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