The Antioxidant Effect of Natural Antimicrobials in Shrimp Primary Intestinal Cells Infected with Nematopsis messor

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dc.contributor.authorBalta, Igori
dc.contributor.authorStef, Lavinia
dc.contributor.authorButucel, Eugenia
dc.contributor.authorPircalabioru, Gratiela Gradisteanu
dc.contributor.authorVenig, Adelina
dc.contributor.authorWard, Patrick
dc.contributor.authorDeshaies, Myriam
dc.contributor.authorPet, Ioan
dc.contributor.authorStef, Ducu
dc.contributor.authorKoyun, Osman Y.
dc.contributor.authorCallaway, Todd R.
dc.contributor.authorGundogdu, Ozan
dc.contributor.authorCorcionivoschi, Nicolae
dc.date.accessioned2022-05-31T15:31:03Z
dc.date.available2022-05-31T15:31:03Z
dc.date.issued2022-05-15
dc.descriptionPublication history: Accepted - 12 May 2022; Published - 15 May 2022.en_US
dc.description.abstractNematopsis messor infections severely impact on shrimp’s health with devastating economic consequences on shrimp farming. In a shrimp primary intestinal cells (SGP) model of infection, a sub-inhibitory concentration (0.5%) of natural antimicrobials (Aq) was able to reduce the ability of N. messor to infect (p < 0.0001). To prevent N. messor infection of SGP cells, Aq inhibits host actin polymerization and restores tight junction integrity (TEER) and the expression of Zo-1 and occluding. The oxidative burst, caused by N. messor infection, is attenuated by Aq through the inhibition of NADPH-produced H2O2. Simultaneous to the reduction in H2O2 released, the activity of catalase (CAT) and superoxide dismutase (SOD) were also significantly increase (p < 0.0001). The antimicrobial mixture inactivates the ERK signal transduction pathway by tyrosine dephosphorylation and reduces the expression of DCR2, ALF-A, and ALF-C antimicrobial peptides. The observed in vitro results were also translated in vivo, whereby the use of a shrimp challenge test, we show that in N. messor infected shrimp the mortality rate was 68% compared to the Aq-treated group where the mortality rate was maintained at 14%. The significant increase in CAT and SOD activity in treated and infected shrimp suggested an in vivo antioxidant role for Aq. In conclusion, our study shows that Aq can efficiently reduce N. messor colonization of shrimp’s intestinal cells in vitro and in vivo and the oxidative induced cellular damage, repairs epithelial integrity, and enhances gut immunityen_US
dc.description.sponsorshipThis study was supported by a grant awarded to NC by Environtech, Dublin, Ireland.en_US
dc.identifierhttp://hdl.handle.net/20.500.12518/442
dc.identifier.citationBalta, I., Stef, L., Butucel, E., Gradisteanu Pircalabioru, G., Venig, A., Ward, P., Deshaies, M., Pet, I., Stef, D., Koyun, O.Y., Callaway, T.R., Gundogdu, O. and Corcionivoschi, N. (2022) ‘The Antioxidant Effect of Natural Antimicrobials in Shrimp Primary Intestinal Cells Infected with Nematopsis messor’, Antioxidants. MDPI AG. doi:10.3390/antiox11050974.en_US
dc.identifier.issn2076-3921 (electronic)
dc.identifier.urihttps://doi.org/10.3390/antiox11050974
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rights© 2022 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).en_US
dc.subjectnatural antimicrobialsen_US
dc.subjectantioxidant capacityen_US
dc.subjectNematopsis messoren_US
dc.subjectintestinal primary cellsen_US
dc.subjecthydrogen peroxideen_US
dc.titleThe Antioxidant Effect of Natural Antimicrobials in Shrimp Primary Intestinal Cells Infected with Nematopsis messoren_US
dc.typeArticleen_US
dcterms.dateAccepted2022-05-12
dcterms.dateSubmitted2022-04-29

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