Elucidation of the Host Bronchial Lymph Node miRNA Transcriptome Response to Bovine Respiratory Syncytial Virus

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dc.contributor.authorJohnston, Dayle
dc.contributor.authorEarley, Bernadette
dc.contributor.authorMcCabe, Matthew S.
dc.contributor.authorKim, Jaewoo
dc.contributor.authorTaylor, Jeremy F.
dc.contributor.authorLemon, Ken
dc.contributor.authorMcMenamy, Michael
dc.contributor.authorDuffy, Catherine
dc.contributor.authorCosby, S. Louise
dc.contributor.authorWaters, Sinéad M.
dc.date.accessioned2021-04-28T14:18:05Z
dc.date.available2021-04-28T14:18:05Z
dc.date.issued2021-04-22
dc.descriptionPublication history: Accepted - 19 March 2021; Published - 22 April 2021.en_US
dc.description.abstractBovine respiratory disease (BRD) causes substantial morbidity and mortality, affecting cattle of all ages. One of the main causes of BRD is an initial inflammatory response to bovine respiratory syncytial virus (BRSV). MicroRNAs are novel and emerging noncoding small RNAs that regulate many biological processes and are implicated in various inflammatory diseases. The objective of the present study was to elucidate the changes in the bovine bronchial lymph node miRNA transcriptome in response to BRSV following an experimental viral challenge. Holstein-Friesian calves were either administered a challenge dose of BRSV (103:5 TCID50/ml 15 ml) (n = 12) or were mock inoculated with sterile phosphate buffered saline (n = 6). Daily scoring of clinical signs was performed and calves were euthanized at day 7 post-challenge. Bronchial lymph nodes were collected for subsequent RNA extraction and sequencing (75 bp). Read counts for known miRNAs were generated using the miRDeep2 package using the UMD3.1 reference genome and the bovine mature miRNA sequences from the miRBase database (release 22). EdgeR was used for differential expression analysis and Targetscan was used to identify target genes for the differentially expressed (DE) miRNAs. Target genes were examined for enriched pathways and gene ontologies using Ingenuity Pathway Analysis (Qiagen). Multi-dimensional scaling (MDS) based on miRNA gene expression changes, revealed a clearly defined separation between the BRSV challenged and control calves, although the clinical manifestation of disease was only mild. One hundred and nineteen DE miRNAs (P < 0.05, FDR < 0.1, fold change > 1.5) were detected between the BRSV challenged and control calves. The DE miRNAs were predicted to target 465 genes which were previously found to be DE in bronchial lymph node tissue, between these BRSV challenged and control calves. Of the DE predicted target genes, 455 had fold changes that were inverse to the corresponding DE miRNAs. There were eight enriched pathways among the DE predicted target genes with inverse fold changes to their corresponding DE miRNA including: granulocyte and agranulocyte adhesion and diapedesis, interferon signalling and role of pathogen recognition receptors in recognition of bacteria and viruses. Functions predicted to be increased included: T cell response, apoptosis of leukocytes, immune response of cells and stimulation of cells. Pathogen recognition and proliferation of cytotoxic T cells are vital for the recognition of the virus and its subsequent elimination.en_US
dc.description.sponsorshipThis project was funded by the Irish Department of Agriculture and the Department of Agriculture, Environment and Rural Affairs, Northern Ireland, as part of the United States-Ireland R&D partnership call (RMIS_0033 Project 16/RD/US-ROI/11). JT and JK were supported by grant number 2017-67015-26760 from the United States Department for Agriculture National Institute for Food and Agriculture.en_US
dc.identifierhttp://hdl.handle.net/20.500.12518/248
dc.identifier.citationJohnston, D., Earley, B., McCabe, M. S., Kim, J., Taylor, J. F., Lemon, K., McMenamy, M., Duffy, C., Cosby, S. L. and Waters, S. M. (2021) ‘Elucidation of the Host Bronchial Lymph Node miRNA Transcriptome Response to Bovine Respiratory Syncytial Virus’, Frontiers in Genetics. Frontiers Media SA, 12. doi: 10.3389/fgene.2021.633125.en_US
dc.identifier.issn1664-8021
dc.identifier.urihttps://doi.org/10.3389/fgene.2021.633125
dc.language.isoenen_US
dc.publisherFrontiers Mediaen_US
dc.rightsCopyright © 2021 Johnston, Earley, McCabe, Kim, Taylor, Lemon, McMenamy, Duffy, Cosby and Waters. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.subjectmiRNAen_US
dc.subjectsmall RNA-Seqen_US
dc.subjectdairy calvesen_US
dc.subjectpneumoniaen_US
dc.subjectbovine respiratory diseaseen_US
dc.subjectBRSV challengeen_US
dc.titleElucidation of the Host Bronchial Lymph Node miRNA Transcriptome Response to Bovine Respiratory Syncytial Virusen_US
dc.typeArticleen_US

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