Evidence for local and international spread of Mycobacterium avium subspecies paratuberculosis through whole genome sequencing of isolates from the island of Ireland

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dc.contributor.authorPerets, Viktor
dc.contributor.authorAllen, Adrian
dc.contributor.authorCrispell, Joseph
dc.contributor.authorCassidy, Sophie
dc.contributor.authorO'Connor, Aoife
dc.contributor.authorFarrell, Damien
dc.contributor.authorBrowne, John A.
dc.contributor.authorO'Mahony, Jim
dc.contributor.authorSkuce, Robin A.
dc.contributor.authorKenny, Kevin
dc.contributor.authorGordon, Stephen V.
dc.date.accessioned2022-04-28T08:03:45Z
dc.date.available2022-04-28T08:03:45Z
dc.date.issued2022-04-05
dc.descriptionPublication history: Accepted - 1 April 2022; Published online - 5 April 2022en_US
dc.description.abstractWe describe application of whole genome sequencing (WGS) to a collection of 197 Mycobacterium avium subsp paratuberculosis (MAP) isolates gathered from 122 cattle herds across 27 counties of the island of Ireland. We compare WGS to MAP diversity quantified using mycobacterial interspersed random unit – variable number tandem repeats (MIRU-VNTR). While MIRU-VNTR showed only two major types, WGS could split the 197 isolates into eight major groups. We also found six isolates corresponding to INMV 13, a novel MIRU-VNTR type for Ireland. Evidence for dispersal of MAP across Ireland via cattle movement could be discerned from the data, with mixed infections present in several herds. Furthermore, comparisons of MAP WGS data from Ireland to data from Great Britain and continental Europe revealed many instances of close genetic similarity and hence evidence for international transmission of infection. BEAST MASCOT structured coalescent analyses, with relaxed and strict molecular clocks, estimated the substitution rate to be 0.10–0.13 SNPs/site/year and disclosed greater transitions per lineage per year from Europe to Ireland, indicating transmission into Ireland. Our work therefore reveals new insight into the seeding of MAP infection across Ireland, highlighting how WGS can inform policy formulation to ultimately control MAP transmission at local, national and international scales.en_US
dc.description.sponsorshipWe acknowledge funding from the Department of Agriculture, Food and Marine awards 15/S/651 (‘NexusMAP’) and 2019R404 (’BTBGe- nIE’).en_US
dc.identifierhttp://hdl.handle.net/20.500.12518/431
dc.identifier.citationPerets, V., Allen, A., Crispell, J., Cassidy, S., O’Connor, A., Farrell, D., Browne, J.A., O’Mahony, J., Skuce, R., Kenny, K. and Gordon, S.V. (2022) ‘Evidence for local and international spread of Mycobacterium avium subspecies paratuberculosis through whole genome sequencing of isolates from the island of Ireland’, Veterinary Microbiology. Elsevier BV. doi:10.1016/j.vetmic.2022.109416.en_US
dc.identifier.issn0378-1135
dc.identifier.urihttps://doi.org/10.1016/j.vetmic.2022.109416
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)en_US
dc.subjectWhole genome sequencingen_US
dc.subjectMycobacterium avium subsp. paratuberculosisen_US
dc.subjectJohne's diseaseen_US
dc.subjectIrelanden_US
dc.titleEvidence for local and international spread of Mycobacterium avium subspecies paratuberculosis through whole genome sequencing of isolates from the island of Irelanden_US
dc.typeArticleen_US
dcterms.dateAccepted2022-04-01
dcterms.dateSubmitted2021-07-13

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