Bioinformatic Analysis of the Campylobacter jejuni Type VI Secretion System and Effector Prediction

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dc.contributor.authorRobinson, Luca
dc.contributor.authorLiaw, Janie
dc.contributor.authorOmole, Zahra
dc.contributor.authorXia, Dong
dc.contributor.authorvan Vliet, Arnoud H.M.
dc.contributor.authorCorcionivoschi, Nicolae
dc.contributor.authorHachani, Abderrahman
dc.contributor.authorGundogdu, Ozan
dc.date.accessioned2021-08-10T10:31:35Z
dc.date.available2021-08-10T10:31:35Z
dc.date.issued2021-06-29
dc.descriptionPublication history: Accepted - 7 June 2021; Published online - 29 June 2021.en_US
dc.description.abstractThe Type VI Secretion System (T6SS) has important roles relating to bacterial antagonism, subversion of host cells, and niche colonisation. Campylobacter jejuni is one of the leading bacterial causes of human gastroenteritis worldwide and is a commensal coloniser of birds. Although recently discovered, the T6SS biological functions and identities of its effectors are still poorly defined in C. jejuni. Here, we perform a comprehensive bioinformatic analysis of the C. jejuni T6SS by investigating the prevalence and genetic architecture of the T6SS in 513 publicly available genomes using C. jejuni 488 strain as reference. A unique and conserved T6SS cluster associated with the Campylobacter jejuni Integrated Element 3 (CJIE3) was identified in the genomes of 117 strains. Analyses of the T6SS-positive 488 strain against the T6SS-negative C. jejuni RM1221 strain and the T6SS-positive plasmid pCJDM202 carried by C. jejuni WP2-202 strain defined the “T6SS-containing CJIE3” as a pathogenicity island, thus renamed as Campylobacter jejuni Pathogenicity Island-1 (CJPI-1). Analysis of CJPI-1 revealed two canonical VgrG homologues, CJ488_0978 and CJ488_0998, harbouring distinct C-termini in a genetically variable region downstream of the T6SS operon. CJPI-1 was also found to carry a putative DinJ-YafQ Type II toxin-antitoxin (TA) module, conserved across pCJDM202 and the genomic island CJIE3, as well as several open reading frames functionally predicted to encode for nucleases, lipases, and peptidoglycan hydrolases. This comprehensive in silico study provides a framework for experimental characterisation of T6SS-related effectors and TA modules in C. jejuni.en_US
dc.identifierhttp://hdl.handle.net/20.500.12518/339
dc.identifier.citationRobinson, L., Liaw, J., Omole, Z., Xia, D., van Vliet, A. H. M., Corcionivoschi, N., Hachani, A. and Gundogdu, O. (2021) ‘Bioinformatic Analysis of the Campylobacter jejuni Type VI Secretion System and Effector Prediction’, Frontiers in Microbiology, 12. doi: 10.3389/fmicb.2021.694824.en_US
dc.identifier.issn1664-302X
dc.identifier.urihttps://doi.org/10.3389/fmicb.2021.694824
dc.language.isoenen_US
dc.publisherFrontiers Mediaen_US
dc.rights© 2021 Robinson, Liaw, Omole, Xia, van Vliet, Corcionivoschi, Hachani and Gundogdu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.subjectCampylobacter jejunien_US
dc.subjectType VI Secretion Systemen_US
dc.subjectT6SS effectorsen_US
dc.subjectT6SS immunity proteinsen_US
dc.subjecttoxin-antitoxinen_US
dc.subjectpathogenicity islanden_US
dc.titleBioinformatic Analysis of the Campylobacter jejuni Type VI Secretion System and Effector Predictionen_US
dc.typeArticleen_US
dcterms.dateAccepted2021-06-07
dcterms.dateSubmitted2021-04-13

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