Diagnosis of sheep fasciolosis caused by Fasciola hepatica using cathepsin L enzyme-linked immunosorbent assays (ELISA)

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dc.contributor.authorLopez Corrales, Jesús
dc.contributor.authorCwiklinski, Krystyna
dc.contributor.authorDe Marco Verissimo, Carolina
dc.contributor.authorDorey, Amber
dc.contributor.authorLalor, Richard
dc.contributor.authorJewhurst, Heather
dc.contributor.authorMcEvoy, Amanda
dc.contributor.authorDiskin, Michael
dc.contributor.authorDuffy, Catherine
dc.contributor.authorCosby, S. Louise
dc.contributor.authorKeane, Orla M.
dc.contributor.authorDalton, John Pius
dc.date.accessioned2021-09-17T15:00:02Z
dc.date.available2021-09-17T15:00:02Z
dc.date.issued2021-07-06
dc.descriptionPublication history: Accepted - 3 July 2021; Published online - 6 July 2021.en_US
dc.description.abstractFasciolosis, a global parasitic disease of agricultural livestock, is caused by the liver fluke Fasciola hepatica. Management and strategic control of fasciolosis on farms depends on early assessment of the extent of disease so that control measures can be implemented quickly. Traditionally, this has relied on the detection of eggs in the faeces of animals, a laborious method that lacks sensitivity, especially for sub-clinical infections, and identifies chronic infections only. Enzyme linked immunosorbent assays (ELISA) offer a quicker and more sensitive serological means of diagnosis that could detect early acute infection before significant liver damage occurs. The performance of three functionally-active recombinant forms of the major F. hepatica secreted cathepsins L, rFhCL1, rFhCL2, rFhCL3, and a cathepsin B, rFhCB3, were evaluated as antigens in an indirect ELISA to serologically diagnose liver fluke infection in experimentally and naturally infected sheep. rFhCL1 and rFhCL3 were the most effective of the four antigens detecting fasciolosis in sheep as early as three weeks after experimental infection, at least five weeks earlier than both coproantigen and faecal egg tests. In addition, the rFhCL1 and rFhCL3 ELISAs had a very low detection limit for liver fluke in lambs exposed to natural infection on pastures and thus could play a major role in the surveillance of farms and a ‘test and treat’ approach to disease management. Finally, antibodies to all three cathepsin L proteases remain high throughout chronic infection but decline rapidly after drug treatment with the flukicide, triclabendazole, implying that the test may be adapted to trace the effectiveness of drug treatment.en_US
dc.description.sponsorshipThis work was supported by a European Research Council Advanced Grant (HELIVAC, 322725) and Science Foundation Ireland (SFI) Professorship grant (17/RP/5368) awarded to J.P. Dalton.en_US
dc.identifierhttp://hdl.handle.net/20.500.12518/352
dc.identifier.citationLópez Corrales, J., Cwiklinski, K., De Marco Verissimo, C., Dorey, A., Lalor, R., Jewhurst, H., McEvoy, A., Diskin, M., Duffy, C., Cosby, S. L., Keane, O. M. and Dalton, J. P. (2021) ‘Diagnosis of sheep fasciolosis caused by Fasciola hepatica using cathepsin L enzyme-linked immunosorbent assays (ELISA)’, Veterinary Parasitology, 298, p. 109517. doi: 10.1016/j.vetpar.2021.109517.en_US
dc.identifier.issn0304-4017
dc.identifier.urihttps://doi.org/10.1016/j.vetpar.2021.109517
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2021 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).en_US
dc.subjectFasciola hepaticaen_US
dc.subjectDiagnosticsen_US
dc.subjectELISAen_US
dc.subjectCoproantigenen_US
dc.subjectCathepsin L peptidasesen_US
dc.titleDiagnosis of sheep fasciolosis caused by Fasciola hepatica using cathepsin L enzyme-linked immunosorbent assays (ELISA)en_US
dc.typeArticleen_US
dcterms.dateAccepted2021-07-03
dcterms.dateSubmitted2021-05-07

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