Natural Antimicrobials Block the Host NF-κB Pathway and Reduce Enterocytozoon hepatopenaei Infection Both In Vitro and In Vivo

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dc.contributor.authorBundurus, Iulia Adelina
dc.contributor.authorBalta, Igori
dc.contributor.authorButucel, Eugenia
dc.contributor.authorCallaway, Todd
dc.contributor.authorPopescu, Cosmin Alin
dc.contributor.authorIancu, Tiberiu
dc.contributor.authorPet, Ioan
dc.contributor.authorStef, Lavinia
dc.contributor.authorCorcionivoschi, Nicolae
dc.contributor.departmentAnimal Health and Welfare
dc.date.accessioned2023-09-04T13:30:12Z
dc.date.available2023-09-04T13:30:12Z
dc.date.issued2023-07-20
dc.descriptionPublication history: Accepted - 19 July 2023; Published - 20 July 2023.
dc.description.abstractThe objective of this work was to investigate, for the first time, the antioxidant effect of a mixture of natural antimicrobials in an Enterocytozoon hepatopenaei (EHP) shrimp-gut model of infection and the biological mechanisms involved in their way of action. The study approach included investigations, firstly, in vitro, on shrimp-gut primary (SGP) epithelial cells and in vivo by using EHP-challenged shrimp. Our results show that exposure of EHP spores to 0.1%, 0.5%, 1%, and 2% AuraAqua (Aq) significantly reduced spore activity at all concentrations but was more pronounced after exposure to 0.5% Aq. The Aq was able to reduce EHP infection of SGP cells regardless of cells being pretreated or cocultured during infection with Aq. The survivability of SGP cells infected with EHP spores was significantly increased in both scenarios; however, a more noticeable effect was observed when the infected cells were pre-exposed to Aq. Our data show that infection of SGP cells by EHP activates the host NADPH oxidases and the release of H2O2 produced. When Aq was used during infection, a significant reduction in H2O2 was observed concomitant with a significant increase in the levels of CAT and SOD enzymes. Moreover, in the presence of 0.5% Aq, the overproduction of CAT and SOD was correlated with the inactivation of the NF-κB pathway, which, otherwise, as we show, is activated upon EHP infection of SGP cells. In a challenge test, Aq was able to significantly reduce mortality in EHP-infected shrimp and increase the levels of CAT and SOD in the gut tissue. Conclusively, these results show, for the first time, that a mixture of natural antimicrobials (Aq) can reduce the EHP-spore activity, improve the survival rates of primary gut-shrimp epithelial cells and reduce the oxidative damage caused by EHP infection. Moreover, we show that Aq was able to stop the H2O2 activation of the NF-κB pathway of Crustins, Penaeidins, and the lysozyme, and the CAT and SOD activity both in vitro and in a shrimp challenge test.
dc.description.sponsorshipWe acknowledge Environtech, Dublin, Ireland for funding the PhD of Eugenia Butucel. Grant number 49650. The publication of this paper was supported through the University of Life Sciences King Mihai I from Timisoara doctoral grants.
dc.identifierhttps://hdl.handle.net/20.500.12518/577
dc.identifier.citationBunduruș, I.A., Balta, I., Butucel, E., Callaway, T., Popescu, C.A., Iancu, T., Pet, I., Stef, L. and Corcionivoschi, N. (2023) ‘Natural Antimicrobials Block the Host NF-κB Pathway and Reduce Enterocytozoon hepatopenaei Infection Both In Vitro and In Vivo’, Pharmaceutics. MDPI AG. Available at: https://doi.org/10.3390/pharmaceutics15071994.
dc.identifier.issn1999-4923 (electronic)
dc.identifier.urihttps://doi.org/10.3390/pharmaceutics15071994
dc.language.isoen
dc.publisherMDPI
dc.rights© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
dc.subjectEnterocytozoon hepatopenaei
dc.subjectnatural antimicrobials
dc.subjectshrimp
dc.subjectinfection
dc.subjectNF-κB pathway
dc.titleNatural Antimicrobials Block the Host NF-κB Pathway and Reduce Enterocytozoon hepatopenaei Infection Both In Vitro and In Vivo
dc.typeArticle
dcterms.dateAccepted2023-07-19
dcterms.dateSubmitted2023-06-12
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