Selenium dietary supplement affects rumen bacterial population dynamics and fermentation parameters in Tibetan sheep (Ovis aries) in alpine meadow

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dc.contributor.authorCui, Xiongxiong
dc.contributor.authorWang, Zhaofeng
dc.contributor.authorTan, Yuhui
dc.contributor.authorChang, Shenghua
dc.contributor.authorZheng, Huiru
dc.contributor.authorWang, Haiying
dc.contributor.authorYan, Tianhai
dc.contributor.authorGuru, Tsedan
dc.contributor.authorHou, Fujiang
dc.date.accessioned2022-03-01T15:34:02Z
dc.date.available2022-03-01T15:34:02Z
dc.date.issued2021-07-02
dc.descriptionPublication history: Accepted - 31 May 2021; Published online - 2 July 2021.en_US
dc.description.abstractSelenium (Se) deficiency is a widespread and seasonally chronic phenomenon observed in Tibetan sheep (Ovis aries) traditionally grazed on the Qinghai–Tibet Plateau (QTP). Effects of the dietary addition of Se-enriched yeast (SeY) on the bacterial community in sheep rumen and rumen fermentation were evaluated with the aim of gaining a better understanding of the rumen prokaryotic community. Twenty-four yearling Tibetan rams [initial average body weight (BW) of 31.0 ± 0.64 kg] were randomly divided into four treatment groups, namely, control (CK), low Se (L), medium Se (M), and high Se (H). Each group comprised six rams and was fed a basic diet of fresh forage cut from the alpine meadow, to which SeY was added at prescribed dose rates. This feed trial was conducted for over 35 days. On the final day, rumen fluid was collected using a transesophageal sampler for analyzing rumen pH, NH3-N content, volatile fatty acid (VFA) level, and the rumen microbial community. Our analyses showed that NH3-N, total VFA, and propionate concentrations in the M group were significantly higher than in the other groups (P < 0.05). Both the principal coordinates analysis (PCoA) and the analysis of similarities revealed that the bacterial population structure of rumen differed among the four groups. The predominant rumen bacterial phyla were found to be Bacteroidetes and Firmicutes, and the three dominant genera in all the samples across all treatments were Christensenellaceae R7 group, Rikenellaceae RC9 gut group, and Prevotella 1. The relative abundances of Prevotella 1, Rikenellaceae RC9 gut group, Ruminococcus 2, Lachnospiraceae XPB1014 group, Carnobacterium, and Hafnia-Obesumbacterium were found to differ significantly among the four treatment groups (P < 0.05). Moreover, Tax4fun metagenome estimation revealed that gene functions and metabolic pathways associated with carbohydrate and other amino acids were overexpressed in the rumen microbiota of SeY-supplemented sheep. To conclude, SeY significantly affects the abundance of rumen bacteria and ultimately affects the rumen microbial fermentation.en_US
dc.description.sponsorshipThis research was funded by the Second Tibetan Plateau Scientific Expedition and Research: Grassland Ecosystem and Ecological Animal Husbandry (2019QZKK0302), the Program for Strategic Priority Research Program of the Chinese Academy of Sciences (XDA20100102), the National Natural Science Foundation of China (No. 31672472 and No. 31201821), and Innovative Research Team of Ministry of Education (IRT_17R50).en_US
dc.identifierhttp://hdl.handle.net/20.500.12518/411
dc.identifier.citationCui, X., Wang, Z., Tan, Y., Chang, S., Zheng, H., Wang, H., Yan, T., Guru, T. and Hou, F. (2021) ‘Selenium Yeast Dietary Supplement Affects Rumen Bacterial Population Dynamics and Fermentation Parameters of Tibetan Sheep (Ovis aries) in Alpine Meadow’, Frontiers in Microbiology. Frontiers Media SA. doi:10.3389/fmicb.2021.663945en_US
dc.identifier.issn1664-302X
dc.identifier.urihttps://doi.org/10.3389/fmicb.2021.663945
dc.language.isoenen_US
dc.publisherFrontiers Mediaen_US
dc.rights© 2021 Cui, Wang, Tan, Chang, Zheng, Wang, Yan, Guru and Hou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.subjectTibetan sheepen_US
dc.subjectseleniumen_US
dc.subjectQinghai-Tibet plateauen_US
dc.subjectbacterial communitiesen_US
dc.subjecthigh-throughput sequencingen_US
dc.subjectgrazingen_US
dc.subjectalpine meadow seleniumen_US
dc.subjectrumen bacterial communitiesen_US
dc.titleSelenium dietary supplement affects rumen bacterial population dynamics and fermentation parameters in Tibetan sheep (Ovis aries) in alpine meadowen_US
dc.typeArticleen_US
dcterms.dateAccepted2021-05-31
dcterms.dateSubmitted2021-02-04

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