Associations between Circulating IGF-1 Concentrations, Disease Status and the Leukocyte Transcriptome in Early Lactation Dairy Cows

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dc.contributor.authorWathes, D. Claire
dc.contributor.authorBecker, Frank
dc.contributor.authorBuggiotti, Laura
dc.contributor.authorCrowe, Mark A.
dc.contributor.authorFerris, Conrad
dc.contributor.authorFoldager, Leslie
dc.contributor.authorGrelet, Clément
dc.contributor.authorHostens, Miel
dc.contributor.authorIngvartsen, Klaus L.
dc.contributor.authorMarchitelli, Cinzia
dc.contributor.authorSalavati, Mazdak
dc.contributor.authorSignorelli, Federica
dc.contributor.authorTakeda, Haruko
dc.contributor.authorTang, Lijing
dc.contributor.authorCheng, Zhangrui
dc.contributor.authorGplusE Consortium
dc.date.accessioned2022-03-28T12:09:28Z
dc.date.available2022-03-28T12:09:28Z
dc.date.issued2021-11-25
dc.descriptionPublication history: Accepted - 19 November 2021; Published - 25 November 2021.en_US
dc.description.abstractPeripartum dairy cows commonly experience negative energy balance (EB) and immunosuppression together with high incidences of infectious and metabolic disease. This study investigated mechanisms linking EB status with immune defense in early lactation. Data were collected from multiparous Holstein cows from six herds and leukocyte transcriptomes were analyzed using RNA sequencing. Global gene expression was related to circulating IGF-1 (as a biomarker for EB) by subdividing animals into three groups, defined as IGF-1 LOW (<35 ng/mL, n = 35), MODERATE (35–100 ng/mL, n = 92) or HIGH (>100 ng/mL, n = 43) at 14 ± 4 days in milk (DIM). Differentially expressed genes between groups were identified using CLC Genomics Workbench V21, followed by cluster and KEGG pathway analysis, focusing on the comparison between LOW and HIGH IGF-1 cows. LOW cows were older and had significantly lower dry matter intakes and EB values, whereas HIGH cows produced more milk. During the first 35 DIM, 63% of LOW cows had more than one health problem vs. 26% HIGH cows, including more with clinical mastitis and uterine infections. Gene expression analysis indicated that leukocytes in LOW cows switched energy metabolism from oxidative phosphorylation to aerobic glycolysis (PGM, LDH, and PDK4). Many antimicrobial peptides were up-regulated in LOW cows (e.g., PTX3, DMBT1, S100A8, and S100A9) together with genes associated with inflammation, platelet activation and the complement cascade. HIGH cows had greater expression of genes regulating T and B cell function and the cytoskeleton. Overall, results suggested an ongoing cycle of poor EB and higher infection rates in LOW IGF-1 cows which was reflected in altered leukocyte functionality and reduced milk production.en_US
dc.description.sponsorshipThis project received funding from the European Union’s Seventh Framework Programme (Brussels, Belgium) for research, technological development, and demonstration under grant agreement no. 613689en_US
dc.identifierhttp://hdl.handle.net/20.500.12518/422
dc.identifier.citationWathes, D.C., Becker, F., Buggiotti, L., Crowe, M.A., Ferris, C., Foldager, L., Grelet, C., Hostens, M., Ingvartsen, K.L., Marchitelli, C., Salavati, M., Signorelli, F., Takeda, H., Tang, L. and Cheng, Z. (2021) ‘Associations between Circulating IGF-1 Concentrations, Disease Status and the Leukocyte Transcriptome in Early Lactation Dairy Cows’, Ruminants. MDPI AG. doi:10.3390/ruminants1020012.en_US
dc.identifier.issn2673-933X (electronic)
dc.identifier.urihttps://doi.org/10.3390/ruminants1020012
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsCopyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).en_US
dc.subjectIGF-1en_US
dc.subjectleukocyteen_US
dc.subjectenergy balanceen_US
dc.subjectRNA-seqen_US
dc.subjectdairy cowen_US
dc.subjectantimicrobialen_US
dc.subjectinflammationen_US
dc.subjectmastitisen_US
dc.subjectPTX3en_US
dc.subjectDMBT1en_US
dc.titleAssociations between Circulating IGF-1 Concentrations, Disease Status and the Leukocyte Transcriptome in Early Lactation Dairy Cowsen_US
dc.typeArticleen_US
dcterms.dateAccepted2021-11-19
dcterms.dateSubmitted2021-10-15

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