Emergence of a non-sporulating secondary phenotype in Clostridium (Clostridioides) difficile ribotype 078 isolated from humans and animals

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dc.contributor.authorConnor, M. C.
dc.contributor.authorMcGrath, J. W.
dc.contributor.authorMcMullan, G.
dc.contributor.authorMarks, N.
dc.contributor.authorGuelbenzu, M.
dc.contributor.authorFairley, D. J.
dc.date.accessioned2020-05-29T11:00:25Z
dc.date.available2020-05-29T11:00:25Z
dc.date.issued2019-09-23
dc.descriptionPublication history: Accepted - 27 August 2019, Published - 23 September 2019en_US
dc.description.abstractClostridium (Clostridioides) difficile is a Gram positive, spore forming anaerobic bacterium that is a leading cause of antibiotic associated diarrhoea in the developed world. C. difficile is a genetically diverse species that can be divided into 8 phylogenetically distinct clades with clade 5 found to be genetically distant from all others. Isolates with the PCR ribotype 078 belong to clade 5, and are often associated with C. difficile infection in both humans and animals. Colonisation of animals and humans by ribotype 078 raises questions about possible zoonotic transmission, and also the diversity of reservoirs for ribotype 078 strains within the environment. One of the key factors which enables C. difficile to be a successful, highly transmissible pathogen is its ability to produce oxygen resistant spores capable of surviving harsh conditions. Here we describe the existence of a non-sporulating variant of C. difficile ribotype 078 harbouring mutations leading to premature stop codons within the master regulator, Spo0A. As sporulation is imperative to the successful transmission of C. difficile this study was undertaken to investigate phenotypic characteristics of this asporogenous phenotype with regards to growth rate, antibiotic susceptibility, toxin production and biofilm formation.en_US
dc.description.sponsorshipThe work was supported by the Department for Employment and Learning, Northern Irelanden_US
dc.identifierhttp://hdl.handle.net/20.500.12518/138
dc.identifier.citationConnor, M. C., McGrath, J. W., McMullan, G., Marks, N., Guelbenzu, M. and Fairley, D. J. (2019) ‘Emergence of a non-sporulating secondary phenotype in Clostridium (Clostridioides) difficile ribotype 078 isolated from humans and animals’, Scientific Reports. Springer Science and Business Media LLC, 9(1). doi: 10.1038/s41598-019-50285-y.en_US
dc.identifier.issn2045-2322
dc.identifier.urihttps://doi.org/10.1038/s41598-019-50285-y
dc.language.isoenen_US
dc.publisherNature Researchen_US
dc.rightsOpen Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en_US
dc.subjectbacteriology, pathogensen_US
dc.titleEmergence of a non-sporulating secondary phenotype in Clostridium (Clostridioides) difficile ribotype 078 isolated from humans and animalsen_US
dc.typeArticleen_US
dcterms.dateAccepted2019-08-27
dcterms.dateSubmitted2019-03-06

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