Whole blood transcriptome analysis in dairy calves experimentally challenged with Bovine Herpesvirus 1 (BoHV-1) and comparison to a Bovine Respiratory Syncytial Virus (BRSV) challenge

O'Donoghue, Stephanie
Earley, Bernadette
Johnston, Dayle
McCabe, Matthew S.
Kim, Jae Woo
Taylor, Jeremy F.
Duffy, Catherine
Lemon, Kenneth
McMenamy, Michael
Cosby, S. Louise
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Frontiers Media
Bovine herpesvirus 1 (BoHV-1), is associated with several clinical syndromes in cattle, among which bovine respiratory disease (BRD) is of particular significance. Despite the importance of the disease, there is a lack of information on the molecular response to infection via experimental challenge with BoHV-1. The objective of this study was to investigate the whole-blood transcriptome of dairy calves experimentally challenged with BoHV-1. A secondary objective was to compare the gene expression results between two separate BRD pathogens using data from a similar challenge study with BRSV. Holstein-Friesian calves (mean age (SD) = 149.2 (23.8) days; mean weight (SD) = 174.6 (21.3) kg) were either administered BoHV-1 inoculate (1 × 107 /mL × 8.5 mL) (n = 12) or were mock challenged with sterile phosphate buffered saline (n = 6). Clinical signs were recorded daily from day (d) −1 to d 6 (post-challenge), and whole blood was collected in Tempus RNA tubes on d six post-challenge for RNA-sequencing. There were 488 differentially expressed (DE) genes (p < 0.05, False Discovery rate (FDR) < 0.10, fold change ≥2) between the two treatments. Enriched KEGG pathways (p < 0.05, FDR <0.05); included Influenza A, Cytokine-cytokine receptor interaction and NOD-like receptor signalling. Significant gene ontology terms (p < 0.05, FDR <0.05) included defence response to virus and inflammatory response. Genes that are highly DE in key pathways are potential therapeutic targets for the treatment of BoHV-1 infection. A comparison to data from a similar study with BRSV identified both similarities and differences in the immune response to differing BRD pathogens
Publication history: Accepted - 25 January 2023; Published online - 17 February 2023
BoHV-1 , transcriptome , whole blood , experimental challenge , BRD
O’Donoghue, S., Earley, B., Johnston, D., McCabe, M.S., Kim, J.W., Taylor, J.F., Duffy, C., Lemon, K., McMenamy, M., Cosby, S.L., Morris, D.W. and Waters, S.M. (2023) ‘Whole blood transcriptome analysis in dairy calves experimentally challenged with bovine herpesvirus 1 (BoHV-1) and comparison to a bovine respiratory syncytial virus (BRSV) challenge’, Frontiers in Genetics. Frontiers Media SA. Available at: https://doi.org/10.3389/fgene.2023.1092877.