Whole blood transcriptome analysis in dairy calves experimentally challenged with Bovine Herpesvirus 1 (BoHV-1) and comparison to a Bovine Respiratory Syncytial Virus (BRSV) challenge

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dc.contributor.authorO'Donoghue, Stephanie
dc.contributor.authorEarley, Bernadette
dc.contributor.authorJohnston, Dayle
dc.contributor.authorMcCabe, Matthew S.
dc.contributor.authorKim, Jae Woo
dc.contributor.authorTaylor, Jeremy F.
dc.contributor.authorDuffy, Catherine
dc.contributor.authorLemon, Kenneth
dc.contributor.authorMcMenamy, Michael
dc.contributor.authorCosby, S. Louise
dc.contributor.authorMorris, Derek W.
dc.contributor.authorWaters, Sinead M.
dc.date.accessioned2023-02-28T09:50:32Z
dc.date.available2023-02-28T09:50:32Z
dc.date.issued2023-02-17
dc.descriptionPublication history: Accepted - 25 January 2023; Published online - 17 February 2023
dc.description.abstractBovine herpesvirus 1 (BoHV-1), is associated with several clinical syndromes in cattle, among which bovine respiratory disease (BRD) is of particular significance. Despite the importance of the disease, there is a lack of information on the molecular response to infection via experimental challenge with BoHV-1. The objective of this study was to investigate the whole-blood transcriptome of dairy calves experimentally challenged with BoHV-1. A secondary objective was to compare the gene expression results between two separate BRD pathogens using data from a similar challenge study with BRSV. Holstein-Friesian calves (mean age (SD) = 149.2 (23.8) days; mean weight (SD) = 174.6 (21.3) kg) were either administered BoHV-1 inoculate (1 × 107/mL × 8.5 mL) (n = 12) or were mock challenged with sterile phosphate buffered saline (n = 6). Clinical signs were recorded daily from day (d) −1 to d 6 (post-challenge), and whole blood was collected in Tempus RNA tubes on d six post-challenge for RNA-sequencing. There were 488 differentially expressed (DE) genes (p < 0.05, False Discovery rate (FDR) < 0.10, fold change ≥2) between the two treatments. Enriched KEGG pathways (p < 0.05, FDR <0.05); included Influenza A, Cytokine-cytokine receptor interaction and NOD-like receptor signalling. Significant gene ontology terms (p < 0.05, FDR <0.05) included defence response to virus and inflammatory response. Genes that are highly DE in key pathways are potential therapeutic targets for the treatment of BoHV-1 infection. A comparison to data from a similar study with BRSV identified both similarities and differences in the immune response to differing BRD pathogens.
dc.description.sponsorshipThis project was funded by the Irish Department of Agriculture, Food and the Marine (DAFM) and the Department of Agriculture, Environment and Rural Affairs (DAERA), Northern Ireland, as part of the US-Ireland R&D partnership call (RMIS_0033 Project 16/RD/US-ROI/11). JT and JK were supported by Grant No. 2017-67015-26760 from the United States Department for Agriculture’s National Institute for Food and Agriculture
dc.identifierhttps://hdl.handle.net/20.500.12518/522
dc.identifier.citationO’Donoghue, S., Earley, B., Johnston, D., McCabe, M.S., Kim, J.W., Taylor, J.F., Duffy, C., Lemon, K., McMenamy, M., Cosby, S.L., Morris, D.W. and Waters, S.M. (2023) ‘Whole blood transcriptome analysis in dairy calves experimentally challenged with bovine herpesvirus 1 (BoHV-1) and comparison to a bovine respiratory syncytial virus (BRSV) challenge’, Frontiers in Genetics. Frontiers Media SA. Available at: https://doi.org/10.3389/fgene.2023.1092877.
dc.identifier.issn1664-8021
dc.identifier.urihttps://doi.org/10.3389/fgene.2023.1092877
dc.language.isoen
dc.publisherFrontiers Media
dc.rights© 2023 O’Donoghue, Earley, Johnston, McCabe, Kim, Taylor, Duffy, Lemon, McMenamy, Cosby, Morris and Waters. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.subjectBoHV-1
dc.subjecttranscriptome
dc.subjectwhole blood
dc.subjectexperimental challenge
dc.subjectBRD
dc.titleWhole blood transcriptome analysis in dairy calves experimentally challenged with Bovine Herpesvirus 1 (BoHV-1) and comparison to a Bovine Respiratory Syncytial Virus (BRSV) challenge
dc.typeArticle
dcterms.dateAccepted2023-01-25
dcterms.dateSubmitted2022-11-08

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