A rapid fungal DNA extraction method suitable for PCR screening fungal mutants, infected plant tissue and spore trap samples

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dc.contributor.authorPilo, Paola
dc.contributor.authorTiley, Anna
dc.contributor.authorLawless, Colleen
dc.contributor.authorKarki, Sujit J.
dc.contributor.authorBurke, James
dc.date.accessioned2021-12-15T11:58:04Z
dc.date.available2021-12-15T11:58:04Z
dc.date.issued2021-11-26
dc.descriptionPublication history: Accepted - 19 November 2021; Published online - 26 November 2021.en_US
dc.description.abstractCurrent DNA extraction methods from filamentous fungi take from an hour to a full day. These methods require buffers and tools for grinding fungal tissues. Although commercial extraction kits can reduce the amount of time spent preparing and extracting genomic DNA from fungal samples, these can be expensive. Here we describe a quick and inexpensive sonication technique to extract genomic DNA from filamentous fungi without buffer which can be used to perform PCR in under an hour. DNA was extracted from Zymoseptoria tritici, Fusarium graminearum and Botrytis cinerea in vitro cultures, Z. tritici asexual fruiting bodies and directly from spore trap tapes.en_US
dc.description.sponsorshipScience Foundation Ireland (SFI) 16/SPP/3296 and 15/CDA/3451.en_US
dc.identifierhttp://hdl.handle.net/20.500.12518/379
dc.identifier.citationPilo, P., Tiley, A.M.M., Lawless, C., Karki, S.J., Burke, J. and Feechan, A. (2022) ‘A rapid fungal DNA extraction method suitable for PCR screening fungal mutants, infected plant tissue and spore trap samples’, Physiological and Molecular Plant Pathology. Elsevier BV. doi:10.1016/j.pmpp.2021.101758.en_US
dc.identifier.issn0885-5765
dc.identifier.urihttps://doi.org/10.1016/j.pmpp.2021.101758
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rightsCopyright 2021 the authors. This is an open access article under a Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits distribution and reproduction for non-commercial purposes, provided the author and source are cited.en_US
dc.subjectFungien_US
dc.subjectDNA extractionen_US
dc.subjectPCRen_US
dc.subjectSonicationen_US
dc.subjectSpore trapen_US
dc.titleA rapid fungal DNA extraction method suitable for PCR screening fungal mutants, infected plant tissue and spore trap samplesen_US
dc.typeArticleen_US
dcterms.dateAccepted2021-11-19
dcterms.dateSubmitted2021-09-02

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