A Novel Prototype Biosensor Array Electrode System for Detecting the Bacterial Pathogen Salmonella typhimurium.

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dc.contributor.authorRamasamy, Palaniappan
dc.contributor.authorDakshinamoorthy, Gajalakshmi
dc.contributor.authorJayashree, Shanmugam
dc.contributor.authorPrabhu, Dhamodharan
dc.contributor.authorRajamanikandan, Sundararaj
dc.contributor.authorVelusamy, Palaniyandi
dc.contributor.authorDayanithi, Govindan
dc.contributor.authorHanna, Robert E.B.
dc.date.accessioned2022-06-14T13:38:01Z
dc.date.available2022-06-14T13:38:01Z
dc.date.issued2022-06-04
dc.descriptionPublication history: Accepted - 2 June 2022: Published online - 4 June 2022en_US
dc.description.abstractSalmonellosis caused by Salmonella sp. has long been reported all over the world. Despite the availability of various diagnostic methods, easy and effective detection systems are still required. This report describes a dialysis membrane electrode interface disc with immobilized specific antibodies to capture antigenic Salmonella cells. The interaction of a specific Salmonella antigen with a mouse anti-Salmonella monoclonal antibody complexed to rabbit anti-mouse secondary antibody conjugated with HRP and the substrate o-aminophenol resulted in a response signal output current measured using two electrode systems (cadmium reference electrode and glassy carbon working electrode) and an agilent HP34401A 6.5 digital multimeter without a potentiostat or applied potential input. A maximum response signal output current was recorded for various concentrations of Salmonella viz., 3, 30, 300, 3000, 30,000 and 300,000 cells. The biosensor has a detection limit of three cells, which is very sensitive when compared with other detection sensors. Little non-specific response was observed using Streptococcus, Vibrio, and Pseudomonas sp. The maximum response signal output current for a dialysis membrane electrode interface disc was greater than that for gelatin, collagen, and agarose. The device and technique have a range of biological applications. This novel detection system has great potential for future development and application in surveillance for microbial pathogens.en_US
dc.description.sponsorshipThis research work was financially supported by DRDE (DRDE-P1-2003/Task-11).en_US
dc.identifierhttp://hdl.handle.net/20.500.12518/453
dc.identifier.citationRamasamy, P., Dakshinamoorthy, G., Jayashree, S., Prabhu, D., Rajamanikandan, S., Velusamy, P., Dayanithi, G. and Hanna, R.E.B. (2022) ‘A Novel Prototype Biosensor Array Electrode System for Detecting the Bacterial Pathogen Salmonella typhimurium’, Biosensors. MDPI AG. doi:10.3390/bios12060389.en_US
dc.identifier.issn2079-6374
dc.identifier.urihttps://doi.org/10.3390/bios12060389
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rights© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/)en_US
dc.subjectSalmonella speciesen_US
dc.subjectbiosensoren_US
dc.subjectimmunosensoren_US
dc.subjectdialysis membrane electrode interface discen_US
dc.subjectglassy carbon electrodeen_US
dc.subjectsalmonellosisen_US
dc.titleA Novel Prototype Biosensor Array Electrode System for Detecting the Bacterial Pathogen Salmonella typhimurium.en_US
dc.typeArticleen_US
dcterms.dateAccepted2022-06-02
dcterms.dateSubmitted2022-04-27

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