The invasive land flatworm Arthurdendyus triangulatus has repeated sequences in the mitogenome, extra-long cox2 gene and paralogous nuclear rRNA clusters
Date
2024-04-03
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Nature Research
Abstract
Using a combination of short- and long-reads sequencing, we were able to sequence the complete mitochondrial genome of the invasive ‘New Zealand flatworm’ Arthurdendyus triangulatus (Geoplanidae, Rhynchodeminae, Caenoplanini) and its two complete paralogous nuclear rRNA gene clusters. The mitogenome has a total length of 20,309 bp and contains repetitions that includes two types of tandem-repeats that could not be solved by short-reads sequencing. We also sequenced for the first time the mitogenomes of four species of Caenoplana (Caenoplanini). A maximum likelihood phylogeny associated A. triangulatus with the other Caenoplanini but Parakontikia ventrolineata and Australopacifica atrata were rejected from the Caenoplanini and associated instead with the Rhynchodemini, with Platydemus manokwari. It was found that the mitogenomes of all species of the subfamily Rhynchodeminae share several unusual structural features, including a very long cox2 gene. This is the first time that the complete paralogous rRNA clusters, which differ in length, sequence and seemingly number of copies, were obtained for a Geoplanidae.
Description
Publication history: Accepted - 1 April 2024; Published - 3 April 2024.
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Keywords
Arthurdendyus, Invasive flatworm, Mitogenome, Short- and long-reads sequencing, Tandem repeats, Paralogous rRNA
Citation
Gastineau, R., Lemieux, C., Turmel, M., Otis, C., Boyle, B., Coulis, M., Gouraud, C., Boag, B., Murchie, A.K., Winsor, L. and Justine, J.-L. (2024) ‘The invasive land flatworm Arthurdendyus triangulatus has repeated sequences in the mitogenome, extra-long cox2 gene and paralogous nuclear rRNA clusters’, Scientific Reports. Springer Science and Business Media LLC. Available at: https://doi.org/10.1038/s41598-024-58600-y.