Katongole, ConstantineYan, Tianhai2022-06-142022-06-142022-05-12Katongole, C.B. and Yan, T. (2022) ‘The Effects of Dietary Crude Protein Level on Ammonia Emissions from Slurry from Lactating Holstein-Friesian Cows as Measured in Open-Circuit Respiration Chambers’, Animals. MDPI AG. doi:10.3390/ani12101243.2076-2615https://doi.org/10.3390/ani12101243Publication history: Accepted - 10 May 2022; Published online - 12 May 2022The effect of dietary crude protein (CP) level on ammonia (NH3) emissions from slurry from lactating Holstein-Friesian cows was studied. Twenty-four-hour total collections of faeces and urine were made from 24 lactating Holstein-Friesian cows fed four total mixed rations containing 141, 151, 177, and 201 g CP/kg DM (6 cows/diet). The collected urine and faeces from each cow were combined to form 2 kg duplicate slurry samples (weight/weight; fresh basis) according to the proportions in which they were excreted. NH3 emissions from the slurry samples were measured, during 0–24 and 24–48 h intervals in six open-circuit respiration chambers maintained at two temperatures (8 or 18 °C). NH3 emissions for the 0–24 and 0–48 h intervals, as well as the average daily emissions, increased linearly with increasing dietary CP level. Increasing the temperature from 8 to 18 °C positively affected NH3 emissions, but only for the 0–24 h interval. In situations where direct measurements are impossible, NH3 emissions from slurry can be predicted accurately using equations based on dietary CP level supported by either urinary nitrogen, faeces nitrogen, or both. In summary, increasing dietary CP level linearly increased average daily NH3 emissions from slurry, with a 5.4 g increase for each 10 g increase in dietary CP.en© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).ammonia emissiondietary crude proteinrespiration chamberslurryArticle