Turner, Andrew D.Dean, Karl J.Dhanji-Rapkova, MonikaDall’Ara, SoniaPino, FlorellaMcVey, ClaireHaughey, SimonLogan, NatashaElliott, ChristopherGago-Martinez, AnaLeao, Jose ManuelGiraldez, JorgeGibbs, RyanThomas, KristaPerez-Calderon, RuthFaulkner, DermotMcEneny, HughSavar, VeroniqueReveillon, DamienHess, PhilippArevalo, FabiolaLamas, J. PabloCagide, EvaAlvarez, MercedesAntelo, AlvaroKlijnstra, Mirjam D.Oplatowska-Stachowiak, MichalinaKleintjens, TimSajic, NerminBoundy, Michael J.Maskrey, Benjamin H.Harwood, D. TimGonzalez Jartın, Jesus M.Alfonso, AmparoBotana, Luis2023-03-232023-03-232023-01-06Turner, A.D., Dean, K.J., Dhanji-Rapkova, M., Dall’Ara, S., Pino, F., McVey, C., Haughey, S., Logan, N., Elliott, C., Gago-Martinez, A., Leao, J.M., Giraldez, J., Gibbs, R., Thomas, K., Perez-Calderon, R., Faulkner, D., McEneny, H., Savar, V., Reveillon, D., Hess, P., Arevalo, F., Lamas, J.P., Cagide, E., Alvarez, M., Antelo, A., Klijnstra, M.D., Oplatowska-Stachowiak, M., Kleintjens, T., Sajic, N., Boundy, M.J., Maskrey, B.H., Harwood, D.T., González Jartín, J.M., Alfonso, A. and Botana, L. (2023) ‘Interlaboratory Evaluation of Multiple LC–MS/MS Methods and a Commercial ELISA Method for Determination of Tetrodotoxin in Oysters and Mussels’, Journal of AOAC INTERNATIONAL. Oxford University Press (OUP). Available at: https://doi.org/10.1093/jaoacint/qsad006.1060-32711944-7922 (electronic)https://doi.org/10.1093/jaoacint/qsad006Publication history: Accepted - 29 December 2022; Published - 6 January 2023.Background Given the recent detection of tetrodotoxin (TTX) in bivalve molluscs but the absence of a full collaborative validation study for TTX determination in a large number of shellfish samples, interlaboratory assessment of method performance was required to better understand current capabilities for accurate and reproducible TTX quantitation using chemical and immunoassay methods. Objective The aim was to conduct an interlaboratory study with multiple laboratories, using results to assess method performance and acceptability of different TTX testing methods. Methods Homogenous and stable mussel and oyster materials were assessed by participants using a range of published and in-house detection methods to determine mean TTX concentrations. Data were used to calculate recoveries, repeatability, and reproducibility, together with participant acceptability z-scores. Results Method performance characteristics were good, showing excellent sensitivity, recovery, and repeatability. Acceptable reproducibility was evidenced by HorRat values for all LC–MS/MS and ELISA methods being less than the 2.0 limit of acceptability. Method differences between the LC–MS/MS participants did not result in statistically different results. Method performance characteristics compared well with previously published single-laboratory validated methods and no statistical difference was found in results returned by ELISA in comparison with LC–MS/MS. Conclusion The results from this study demonstrate that current LC–MS/MS methods and ELISA are on the whole capable of sensitive, accurate, and reproducible TTX quantitation in shellfish. Further work is recommended to expand the number of laboratories testing ELISA and to standardize an LC–MS/MS protocol to further improve interlaboratory precision. Highlights Multiple mass spectrometric methods and a commercial ELISA have been successfully assessed through an interlaboratory study, demonstrating excellent performance.enCopyright: The Author(s) 2023. Published by Oxford University Press on behalf of AOAC INTERNATIONAL. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.comArticle