Hijazi, AymanPisano, ItaloLeahy, J.J.Kwapinski, WitoldJohnston, ChristopherPrendergast, John2022-09-222022-09-222022-09-05Hijazi, A., Pisano’, I., Leahy, J.J., Kwapinski, W., Johnston, C.R. and Prendergast, J. (2022) ‘Identification of high-value bioactive constituents in Northern European willow varieties: S.X. Dasyclados, Endeavour, Cheviot, Tora, Resolution, S. Purpurea, Terranova, endurance’, Phytomedicine Plus. Elsevier BV. Available at: https://doi.org/10.1016/j.phyplu.2022.100342.2667-0313https://doi.org/10.1016/j.phyplu.2022.100342Publication history: Accepted - 2 September 2022; Published online - 5 September 2022Willow trees have maintained their place in medicine for many years as they are herbal source of various cures. Willow varieties are considered to contain a wide range of anti-inflammatory and anti-bacterial species such as salicylates and flavonoids. The current work is centred on the presence of high bioactive pharmaceutical constituents other than salicin such as flavan-3-ol catechin, salicortin, and other complex compounds, which contribute to the total medical value of willow extracts. To evaluate the distribution of these bioactives, bark, and wood fractions of 8 different willow varieties (S.X. Dasyclados, Endeavour, Cheviot, Tora, Resolution, S. Purpurea, Terranova, Endurance) were extracted using dispersive solid phase extraction and then analysed using LC-MS comprising a quadrupole time-of-flight spectrometer. Indeed, various high value constituents such as salicortin, catechin, triandrin, acacetin-5-O-xyloside, picein, apigenin-7-O-glucoside, vitexin-2-rhamnoside, luteolin-7-glucoside, catechin gallate, and kaempferol as well as giberellic and 5-methoxysalicylic acid were detected in bark and wood fractions of the willow varieties 80:20 ethanol/water extracts.en© 2022 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).WillowBarkBioactivesPolyphenolsSalicinAnti-inflammatoryArticle