Butucel, EugeniaBalta, IgoriMcCleery, DavidMorariu, FloricaPet, IoanPopescu, Cosmin AlinStef, LaviniaCorcionivoschi, Nicolae2022-09-082022-09-082022-08-18Butucel, E., Balta, I., McCleery, D., Morariu, F., Pet, I., Popescu, C.A., Stef, L. and Corcionivoschi, N. (2022) ‘Farm Biosecurity Measures and Interventions with an Impact on Bacterial Biofilms’, Agriculture. MDPI AG. Available at: https://doi.org/10.3390/agriculture12081251.2077-0472 (electronic)https://doi.org/10.3390/agriculture12081251Publication history: Accepted - 16 August 2022; Published - 18 August 2022.Farm biosecurity management includes a set of practical measures used to prevent and limit the spread of infections to humans and animals. Infections, predominantly caused by zoonotic agents, often occur due to a lack of safety standards monitoring on farms, but also because of the use of inappropriate antimicrobial products leading to bacterial resistance, tolerance to biocides and the emergence antimicrobial-resistant germs. To date, research was mainly focused on studying the antimicrobial resistance in bacterial biofilms and the mechanisms involved in their occurrence. At molecular level, the limited diffusion of biocides in the biofilm matrix, enzyme-mediated resistance, genetic adaptation, efflux pumps, and levels of metabolic activity inside the biofilm are some of the investigated biological mechanisms which can promote antimicrobial resistance in biofilms were also investigated. Interventions, based on the identification of novel antimicrobial compounds, that would exclude the occurrence of bacterial tolerance, including essential oils (oregano, cloves), organic acids (tannic & oleic acid) and natural plant compounds (e.g. alkaloids, flavonoids, tannins and coumarins) were also extensively studied and reviewed given their effectiveness against pathogen-produced biofilms. The aim of this review was emphasize the importance of biosecurity and farm management practices and to assess their impact on bacterial biofilm formation. Furthermore, we present the recent intervention strategies aimed at reducing and combating the formation of bacterial biofilms in livestock farms.enCopyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).farm biosecuritydisinfectioninfection control and preventionanti-biofilm strategiessustainable livestockbiocidespathogensArticle