Pilo, PaolaTiley, AnnaLawless, ColleenKarki, Sujit J.Burke, James2021-12-152021-12-152021-11-26Pilo, P., Tiley, A.M.M., Lawless, C., Karki, S.J., Burke, J. and Feechan, A. (2022) ‘A rapid fungal DNA extraction method suitable for PCR screening fungal mutants, infected plant tissue and spore trap samples’, Physiological and Molecular Plant Pathology. Elsevier BV. doi:10.1016/j.pmpp.2021.101758.0885-5765https://doi.org/10.1016/j.pmpp.2021.101758Publication history: Accepted - 19 November 2021; Published online - 26 November 2021.Current DNA extraction methods from filamentous fungi take from an hour to a full day. These methods require buffers and tools for grinding fungal tissues. Although commercial extraction kits can reduce the amount of time spent preparing and extracting genomic DNA from fungal samples, these can be expensive. Here we describe a quick and inexpensive sonication technique to extract genomic DNA from filamentous fungi without buffer which can be used to perform PCR in under an hour. DNA was extracted from Zymoseptoria tritici, Fusarium graminearum and Botrytis cinerea in vitro cultures, Z. tritici asexual fruiting bodies and directly from spore trap tapes.enCopyright 2021 the authors. This is an open access article under a Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits distribution and reproduction for non-commercial purposes, provided the author and source are cited.FungiDNA extractionPCRSonicationSpore trapArticle