Screening method for the detection of residues of amphenicol antibiotics in bovine milk by optical biosensor
Date
2020-09-10
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor & Francis
Abstract
An immunobiosensor assay was developed for multi-residue screening in bovine milk of the parent
amphenicols, thiamphenicol and florfenicol, along with the metabolite florfenicol amine.
A polyclonal antibody raised in a rabbit after immunisation with a florfenicol amine-protein
conjugate was employed in the assay. Milk samples were subjected to acetonitrile extraction,
reconstituted in buffer and diluted prior to biosensor analysis. Validation data obtained from the
analysis of fortified samples has shown that the method has a detection capability of less than
0.25 μg kg−1 for florfenicol and less than 0.5 μg kg−1 for florfenicol amine and thiamphenicol. The
cross-reactivity profile and validation data for the detection of these amphenicols is presented
together with results obtained following the analysis of florfenicol incurred samples using the
developed screening method along with a comparison of results obtained from the analysis of the
same incurred samples using an MRM3 UPLC-MS/MS confirmatory method. Results are also presented
obtained from the analysis of samples from both treated and non-treated animals which
were co-housed and which show the potential for cross-contamination.
Description
Publication history: Accepted - 16 July 2020; Published online - 10 September 2020.
item.page.type
Article
item.page.format
Keywords
screening, detection, amphenicols, milk, optical biosensor
Citation
Thompson, C. S., Traynor, I. M., Fodey, T. L., Barnes, P., Faulkner, D. V. and Crooks, S. R. H. (2020) ‘Screening method for the detection of residues of amphenicol antibiotics in bovine milk by optical biosensor’, Food Additives & Contaminants: Part A. Informa UK Limited, 37(11), pp. 1854–1864. doi: 10.1080/19440049.2020.1809718.