Screening method for the detection of residues of amphenicol antibiotics in bovine milk by optical biosensor

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dc.contributor.authorThompson, Colin
dc.contributor.authorTraynor, Imelda
dc.contributor.authorFodey, Terence
dc.contributor.authorBarnes, Paul
dc.contributor.authorFaulkner, Dermot
dc.contributor.authorCrooks, Steven
dc.date.accessioned2021-05-04T11:34:07Z
dc.date.available2021-05-04T11:34:07Z
dc.date.issued2020-09-10
dc.descriptionPublication history: Accepted - 16 July 2020; Published online - 10 September 2020.en_US
dc.description.abstractAn immunobiosensor assay was developed for multi-residue screening in bovine milk of the parent amphenicols, thiamphenicol and florfenicol, along with the metabolite florfenicol amine. A polyclonal antibody raised in a rabbit after immunisation with a florfenicol amine-protein conjugate was employed in the assay. Milk samples were subjected to acetonitrile extraction, reconstituted in buffer and diluted prior to biosensor analysis. Validation data obtained from the analysis of fortified samples has shown that the method has a detection capability of less than 0.25 μg kg−1 for florfenicol and less than 0.5 μg kg−1 for florfenicol amine and thiamphenicol. The cross-reactivity profile and validation data for the detection of these amphenicols is presented together with results obtained following the analysis of florfenicol incurred samples using the developed screening method along with a comparison of results obtained from the analysis of the same incurred samples using an MRM3 UPLC-MS/MS confirmatory method. Results are also presented obtained from the analysis of samples from both treated and non-treated animals which were co-housed and which show the potential for cross-contamination.en_US
dc.identifierhttp://hdl.handle.net/20.500.12518/252
dc.identifier.citationThompson, C. S., Traynor, I. M., Fodey, T. L., Barnes, P., Faulkner, D. V. and Crooks, S. R. H. (2020) ‘Screening method for the detection of residues of amphenicol antibiotics in bovine milk by optical biosensor’, Food Additives & Contaminants: Part A. Informa UK Limited, 37(11), pp. 1854–1864. doi: 10.1080/19440049.2020.1809718.en_US
dc.identifier.issn1944-0049
dc.identifier.urihttps://doi.org/10.1080/19440049.2020.1809718
dc.language.isoenen_US
dc.publisherTaylor & Francisen_US
dc.rights© 2020 Taylor & Francis Group, LLC. This work is made available online in accordance with the publisher’s policies. Please refer to any applicable terms of use of the publisher.en_US
dc.subjectscreeningen_US
dc.subjectdetectionen_US
dc.subjectamphenicolsen_US
dc.subjectmilken_US
dc.subjectoptical biosensoren_US
dc.titleScreening method for the detection of residues of amphenicol antibiotics in bovine milk by optical biosensoren_US
dc.typeArticleen_US

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